摘要
目的:探讨氯胺酮连续鞘内注射对慢性坐骨神经损伤大鼠脊髓背角N-甲基-D天冬氨酸亚基(NR2B)mRNA表达的影响。方法:雄性SD大鼠18只,随机分为假手术组、CCI组和氯胺酮组。按Bennett等法制作CCI模型,测von-Frey丝触痛及冷水阈值,采用原位杂交技术检测各组脊髓背角NR2B mRNA表达的变化。结果:CCI组痛阈显著下降,冷水阈显著升高,脊髓背角有大量NR2BmRNA阳性表达(P<0.01);氯胺酮组仅出现轻度痛敏症状,NR2B mRNA表达受到明显抑制(P<0.01)。结论:NR2B mRNA表达上调可能是神经损伤后慢性疼痛的发病机制之一,氯胺酮可抑制其表达从而发挥一定程度的镇痛作用。
Objective： To evaluate the effect of intrathecal ketamine on the expression of NR2B mRNA in the spinal dorsal cord of CCI rats. Methods： Eighteen male Sprague-Dawley rats were randomly divided into 3 groups： In group Ⅰ the animals were shamly operated; In group Ⅱ （CCI） the rats were placed four loose ligatures around the right sciatic nerve; In group Ⅲ （CCI ＋ KET） of ketamine 1 mg/kg was administered 30min before and 1, 2, 3, 5, 7, 9, 11 days after operation. Rats were tested for hyperalgesia by measuring paw withdrawal latency （PWL） using von-Frey filaments and ice-cold water. The changes in NR2B mRNA expression in the spinal cord were detected by hybridization in situ technique. Results： The expression of NR2B mRNA was minimal in sham operation group; CCI induced significant increase in the expression of NR2B mRNA in the ipsilateral spinal cord （P 〈 0.01）, ketamine i.t. significantly inhibited the increase in NR2B mRNA expression （P 〈 0.01）. Conclusion： Upregulation of NR2B expression may be involved in the development of neuropathic pain, and inhibition of NR2B expression may contribute to the antinociceptive effects of ketamine.
出处
《中国疼痛医学杂志》
CAS
CSCD
北大核心
2005年第5期290-293,共4页
Chinese Journal of Pain Medicine
基金
国家自然科学基金资助项目(NO.20302015)
