摘要
研究不同剂量γ射线照射及γ射线和丝裂霉素C(MitomycinC,MMC)联合用药对雄性SD(Sprague-Dawley)大鼠肝脏微粒体中CYP2B1、CYP2E1的影响。从空白组(未经苯巴比妥诱导)和经苯巴比妥(PB)诱导组大鼠的肝脏中提出微粒体,分别进行2Gy、8Gy和16Gyγ射线单纯照射;50μmol/LMMC单纯处理;γ射线照射后加MMC联合作用,不作任何处理的作为正常对照组,然后测定CYP2B1、CYP2E1活性。实验结果显示,无论是空白组,还是PB诱导组的微粒体,在各剂量γ射线照射后,CYP2B1、CYP2E1活性变化都没有统计学差异(p>0.05);50μmol/LMMC作用后,CYP2B1活性没有明显变化(p>0.05),但使CYP2E1活性下降明显(p<0.01)。两因素析因分析发现对于微粒体中CYP2B1、CYP2E1活性,γ射线照射和MMC作用之间没有交互作用。说明在体外试验中,γ照射对大鼠肝脏微粒体CYP2B1、CYP2E1活性没有影响,50μmol/LMMC的作用对CYP2B1活性也没有影响,但可以抑制CYP2E1活性。
The purpose of this study is to evaluate activity changes of CYP2B l and CYP2E1 in male SD rat hepatic microsomes by γ-irradiation and/or MMC in vitro. The hepatic microsomes from blank control or phenobarital （PB）-induced rats were treated by^137 Cs γ-rays （2Gy, 8Gy and 16Gy）, 50μmol/L MMC, or 50μmol/L MMC after the irradiation The CYP2B1 and CYP2E1 activities were determined, and were compared with the control group, which were untreated.. The results showed that the CYP2B1 and CYP2E1 activities were not changed after the irradiation in PB-induced rat liver microsomes （P〉0.05）; The CYP2E1 activity decreased significantly by the MMC treatment （P〈0.01）, whereas the CYP2B1 activity did not changed （P〉0.05）. There was no interaction between MMC and γ-irradiation on the CYP2B1 and CYP2E1 activities. In conclusion, the present investigations indicated that the CYP2E1 activity was inhibited significantly in 50μmol/L MMC, but not in γ-irradiation in vitro. The CYP2B1 activity waS no significant changed not onlv under γ-irradiation but also MMC treatment in vitro.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2005年第5期312-315,共4页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金(30100042)资助
