商陆培养细胞对6β-santonin的生物转化(英文)

Biotransformation of 6β-santonin by Phytolacca acinosa cell suspension cultures

目的利用商陆培养细胞对6βsantonin(1)进行生物转化,以获得多种利于进一步进行结构改造的衍生物。方法将底物1与商陆培养细胞共孵化,采用色谱分离技术得到产物,通过理化性质和光谱数据对产物进行结构鉴定。结果与商陆培养细胞共孵化7d后,底物1转化为5个产物(2～6),其中3为新化合物。结论利用商陆培养细胞可对6βsantonin进行选择性还原及羟基化反应,可为其进一步化学结构改造提供有价值的中间体。

Aim To obtain more valuable derivatives for the further structural modification of 6β- santonin （1） via biotransformation by using cell suspension cultures of Phytolacca acinosa. Methods The substrate 1 was incubated with cell suspension cultures of P. acinosa, the products were obtained by chromatography, and identified on the basis of their physical and spectral data （HRMS, 1D NMR, 2D NMR, NOE and IR ）. Results After incubation with cell suspension cultures of P. acinosa, 1 was converted into five products （2-6）. Among them, 3 is a new compound. Conclusion 6β-santonin could be selectively reduced and hydroxylated by the cell suspension cultures of P. acinosa, which would provide valuable intermediates for its further structural modification.