高效液相色谱-大气压化学电离质谱联用分析天然酮甙类化合物

Analysis of Natural Xanthone Glycosides by High Performance Liquid Chromatography/Atmosphere Pressure Chemical Ionization Mass Spectrometry

利用高效液相色谱大气压化学电离质谱联用技术,探讨了酮单糖甙和酮双糖甙在负离子模式下的质谱断裂特点,比较相同条件下不同酮甙的质谱图,借助[Y0-1]-.和Y0-离子的相对丰度关系探讨了糖基的性质对断裂规律的影响。同时借助[Y0-1]-.、Y1-及Z1-离子的丰度实现对双糖基为1→2与1→6连接的酮双糖甙的区分。

High performance liquid chromatography/atmosphere pressure chemical ionization mass spectrometry （MS） was used to study the collision-induced dissociation （CID） of O-glycoside bond of xanthone glycosides. The fragmentation of O-glycosides, C-glycoside and O-diglycosides in negative ion mode was studied. By comparing with the spectra of different compounds under the same MS condition , it can be deduced that the relative abundance of the radical aglycone （ [Y0-1 ]^- ） and aglycone （ Y0^- ） was affected by the nature of the glycan. A clear differentiation between a 1→2 linkage and 1→6 linkage in the sugar part was obtained by [A0-1] , Y1 and Z1.