不同器官细胞因子在多细菌感染性炎症时的基因表达和临床意义

Changes and significance of cytokine gene expression in different organs with multiple microbacterial peritonitis

目的探讨肝、肺细胞因子基因表达与腹腔吞噬细胞上清液、循环血中细胞因子含量的关系,为临床诊治多细菌感染引发的炎症提供实验依据。方法将30只小鼠分为假手术对照组(sham组)和盲肠结扎组(CLP组)。采用RT!PCR法检测肝脏和肺脏肿瘤坏死因子α(TNF!α)和白细胞介素10(IL!10)的基因表达情况,采用ELISA法检测腹腔巨噬细胞上清液和循环血液中相应细胞因子含量。结果CLP组的TNF"α、IL!10基因表达和腹腔巨噬细胞上清液、循环血液中的相应细胞因子含量均高于sham组。CLP后18h组与4h组比较,TNF"α在腹腔巨噬细胞上清液、循环血液中的活性均有显著性差异(P<0.05),在肝、肺中基因表达有非常显著性差异(P<0.01);IL!10在腹腔巨噬细胞上清液和循环血液中的含量无显著性差异,而在肝、肺中基因表达有显著性差异。结论发生多细菌感染性炎症时,肝、肺参与细胞因子的表达;血液中细胞因子含量不能完全代表组织器官内的基因表达情况;多细菌感染性炎症治疗应考虑靶器官细胞因子的表达状态。

Objective To investigate the correlation between levels of cytokines gene expression in the liver, lungs and the concentrations of cytokines in sera and the supernatant fluid of peritoneal macrophages in mice by cecal ligation and puncture（CLP）. Methods 30 C57BL/6 mice were randomly divided into sham group （control group） and CLP group. RNA from the liver and lungs were isolated to determine the expression of TNF-α and IL-10 mRNA by reverse transcription polymerase chain reaction（RT-PCR） . ELISA was used to measure the concentrations of TNF-α and IL-10 in sera and supernatant fluid of peritoneal macrophages. Results The concentrations of TNF-α and IL-10 in sera and supernatant fluid of peritoneal macrophages and levels of gene expression in liver and lungs were significantly higher in mice with CLP than in those of sham controls. TNF-α gene expression was increased more significant from 4 hrs to 18 hrs both in liver and lungs （P〈0.01）than the concentrations in sera and peritoneal fluid （P〈0.05）. The concentrations of IL-10 in sera and supernatant fluid of peritoneal macrophages were not markedly changed from 4 hrs to 18 hrs, while tge gene expression in liver and lungs were stronger in 18 hrs than in 4 hrs. Conclusions Liver and lungs are organs that produce cytokines, and the change of cytokines in blood cannot represent that in organs of bodies suffered from muhiple-microbarcterial infection.