苦参碱对脂多糖/D-氨基半乳糖诱导的肝炎及离体巨噬细胞释放肿瘤坏死因子的影响(英文)

Effect of matrine on lipopolysaccharides/D-galactosamine-induced hepatitis and tumor necrosis factor release from macrophages in vitro

目的:研究苦参碱(Mat)对脂多糖(lipopolysaccharides,LPS)诱导的D-氨基半乳糖(D-GalN)致敏小鼠致死性肝炎以及腹腔巨噬细胞(PM)释放肿瘤坏死因子(TNF)的影响。方法:小鼠ip Mat10,50mg·kg^(-1),bid×3d,然后ip LPS(1μg·kg^(-1))和D-GalN(800mg·kg^(-1)),通过病理组织学观察及测定血清丙氨酸转氨酶(ALT)活性来评估肝损伤。小鼠PM培养上清中的TNF活性以杀伤L929细胞的结晶紫染色法测定。结果:Mat降低了LPS/D-GalN引起的血清ALT活性升高及小鼠对LPS/D-GalN致死毒性的敏感性并抑制LPS诱导的小鼠PM释放TNF。结论:Mat防治LPS/D-GalN引起的致死性肝炎,并抑制LPS诱导的TNF释放。

AIM: To study the effects of matrine (Mat) on lipopolysaccharides (LPS)-induced fatal hepatitis in D-galactosamine (D-GalN)-sensitized mice and tumor necrosis factor ( TNF ) release from peritoneal macrophages (PM0). METHODS: Mice were pretreated with Mat (10, 50 mg·kg-1, ip, bidx3 d), and then injected ip LPS+D-GalN. Liver injury was assessed by quantifying plasma activity of alanine aminotransferase ( ALT) and histopathological examination. The TNF activities in the supernatants of mouse PM0 stimulated with LPS in the presence of Mat (32.5-500 mg·L-1) were monitored by the L929 target cells lytic assay. RESULTS: Mat pretreatment markedly diminished hepatic injury induced by LPS in combination with D-GalN. Mat inhibited LPS-induced TNF release from mouse PM0 in vitro in a concentration-dependent manner. CONCLUSION: Mat protected the D-GalN-treated mice from the development of fatal hepatitis induced by LPS, and inhibited the LPS-induced TNF release from mouse PMO.