怀化侗族高血压高发人群醛固酮合成酶基因多态性与原发性高血压及血脂水平的关系

Relationship of polymorphism of aldosterone synthase gene with essential hypertension and blood lipids in high-risk population of hypertension in Dong nationality group in Huaihua

目的:探讨醛固酮合成酶基因多态性与怀化侗族高血压高发人群原发性高血压及血脂水平的关系。方法:于2003-06/2004-12对湖南怀化地区通道侗族自治县1个独立自然村落30岁以上人群174人进行分析。以其中原发性高血压89人为侗族高血压组;血压正常人群85人为侗族血压正常组。同期选择怀化地区汉族人群178人,以其中高血压56人为汉族高血压组;以血压正常人群122人为汉族血压正常组。纳入对象均了解本实验目的,并自愿接受。采用聚合酶链反应结合限制性内切酶片段长度多态分析技术检测纳入对象醛固酮合成酶基因-344C/T等位基因频率和基因型频率。采用北京中生公司生产的试剂盒,按说明书要求,用生化自动分析仪检测血清三酰甘油、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖,用电解质分析仪检测血钾、钠、氯、钙。基因型和等位基因频率比较和组间计数资料采用χ2检验。两组均数间比较采用t检验。结果:①侗族高血压和血压正常人群89和85人,汉族高血压和血压正常人群56和122人,均进入结果分析。②-344C/T基因型频率和等位基因频率在侗族高血压高发人群分别为CC0.103,CT0.351,TT0.546和C0.285,T0.715,汉族人群分别为CC0.067,CT0.372,TT0.561和C0.253,T0.747;这两种人群高血压患者和正常人的-344C/T基因型频率和等位基因频率差异不明显。③侗族高血压组和侗族血压正常组人群CC和CT+TT基因型携带者血压水平、血压、血化学指标相近(P>0.05)。④侗族高血压组空腹血糖、三酰甘油、低密度脂蛋白胆固醇、总胆固醇和血钠水平明显高于侗族血压正常组(P<0.05),高密度脂蛋白胆固醇及血钾水平明显低于侗族血压正常组(P<0.05)。结论:①怀化侗族人群醛固酮合成酶基因多态性可能与原发性高血压及生化特征无关。②怀化侗族人群患高血压可能与空腹血糖、三酰甘油、低密度脂蛋白胆固醇、总胆固醇及血钠升高和高密度脂蛋白胆固醇、血钾水平降低及钠与钾比值增高有关。

AIM： To investigate the relationship of polymorphism of aldosterone synthase （CYP11B2） gene with essential hypertension （EH） and blood lipids in the high-risk population of EH in Dong nationality group in Huaihua district. METHODS： 174 people aged over 30 years old from one independent natural village of Dong autonomy county of Huaihua district of HuMan province from June 2003 to December 2004 were analyzed. Among them, 89 patients with EH were selected as Dong EH group, 85 people with normal blood pressure as Dong normal group. 178 HaM nationality people were selected at the same period in the Huaihua district, among them, 56 people with hypertenstion as HaM hypertension group, and 122 with normal blood pressure as HaM blood pressure normal group. All of the included objects understood the aim of the experiment, and accepted it voluntarily. Polymerase chain reaction and restriction fragment length polymorphism （PCR- RFLP） was used for genotyping of allele frequency and genotypic frequency of CYPI IB2-344C/T. The test kits produced by Beijing Zhongsheng Company were used. According to the specification, serum triacylglycerol, total cholesterol, high density lipoprotein-cholesterol （HDL-C）, low density lipeprotein-cholesterol （LDL-C） and fasting glucose were detected by biochemistry automatic analyzer. Electrolure analyzer tested the blood kaiu, natrium, chlorine and calcium. The comparison of frequency of genotype and allele gene and the enumeration data among groups were checked by the A-2 test. The means between the two groups were compared by t-test. RESULTS：① Eighty-nine with hypertension and 85 normal blood pressure people in Dong and 56 with hypertension and 122 normal blood pressure people in HaM were all involved in the result analysis. ②Frequencies of -344C/T genotypes and alleles were CC 0.10.3, CT 0.351, TT 0.546 and C 0.285, T 0.715 in high-risk population with hypertension in Dong, respectively; It was CC 0.067, CT 0.372, TT 0.561 and C 0.253, T 0.747 in HaM, respectively. There was insignificant difference of -344C/T genotypic frequency and allele frequency between the hypertension patients in the two groups and the normal people. ③ The blood pressure level, blood chemical index in patients with CC and CT＋TF genotype in the Dong hypertension group and Dong normal people group were close （P 〉 0.05）. ④ The levels of fasting glucose, triacylglycerol, LDL-C, total cholesterol and blood natrium in Dong hypertension group were significantly higher than those in the Dong normal blood pressure group （P 〈 0.05）, and the levels of HDL-C and blood kaiu were significantly lower than those in the Dong normal blood pressure group （P 〈 0.05）. CONCLUSION： ① The polymorphism of the CYPI IB2 gene may not is associated with EH and the characteristics of its biochemical criteria in the population of Dong nationality group in Huaihua district. ②The hypertension of Dong group in Huanhua maybe has relation with the increase of fasting glucose, triacylglycerol, LDL-C, total cholesterol and the blood natfium and the decrease of HDL-C and blood kaiu and the rise of ratio of natrium and kaiu.