艾司洛尔对大鼠心肌氧自由基代谢的干预效应

Interventional effects of esmolol on metabolism of oxygen-derived free radicals in myocardial tissues of rats

目的:探讨在心肺复苏时应用超短效β1受体阻滞剂艾司洛尔对复苏后心肌氧自由基代谢的影响。方法:实验于2002-01/2003-01在中国医科大学附属第二医院中心实验室完成。选用66只健康雄性Wistar大鼠随机分为3组:假手术组、肾上腺素组、肾上腺素+艾司洛尔组。采用窒息致大鼠再灌注损伤的复苏模型,监测心电图、心率、体温、平均动脉压。观察自主循环恢复后30,120,180min心肌超氧化物歧化酶(抗氧化酶)、丙二醛(脂质过氧化反应的最终产物)的变化。结果:进入结果分析实验动物54只,每组18只,每时点6只。①心肌超氧化物歧化酶活性:肾上腺素组、肾上腺素+艾司洛尔组各时点均低于假手术组犤复苏后30min,120min,180min各组:肾上腺素组为(104.06±6.11),(84.23±5.53),(69.08±8.76)NU/mg,肾上腺素+艾司洛尔组为(116.33±4.85),(99.33±7.93),(79.67±3.82)NU/mg,假手术组为(138.19±7.97),(135.48±6.43),(130.99±4.85)NU/mg,P<0.01犦,肾上腺素+艾司洛尔组高于肾上腺素组(P<0.05)。②心肌丙二醛含量:肾上腺素组、肾上腺素+艾司洛尔组各时点高于假手术组犤复苏后30min,120min,180min各组:肾上腺素组为(15.97±0.99),(21.48±2.84),(33.90±4.39)μmol/g,肾上腺素+艾司洛尔组为(12.88±1.12),(16.04±1.75),(23.59±1.85)μmol/g,假手术组为(10.80±1.43),(12.08±0.76),(12.22±1.84)μmol/g,P<0.01犦,肾上腺素+艾司洛尔组低于假手术组(P<0.01)。结论:艾司洛尔可能通过减轻超氧化物歧化酶活性的降低和丙二醛含量的升高而改善复苏后心肌氧自由基代谢的失衡。

AIM： To explore the effects of the super-short effective β1 receptor blocker esmolol at the moment of cardio-pulmonary resuscitation on oxygen-derived free radicals metabolism in myocardial tissue after resuscitation. METHODS： The experiment was done at the central laboratory of Second Affiliated Hospital, China Medical University from January 2002 to January 2003. Sixty-six healthy male Wistar rats were randomly divided into three groups： sham operation group, adrenaline group, and adrenaline ＋esmolol group. The resuscitation model of rats with reperfusion injury was induced by asphyxiation. ECG, heart rate, temperature and mean arterial blood pressure were measured. The changes of myocardium superoxide dismutase （SOD） （anti-oxidase） and malondialdehyde （the final product of lipid peroxidation reaction） were observed at the 30, 120, 180 minutes after the restoration of spontaneous circulation. RESULTS： Fifty-four experimental animals were involved in the result analysis, 18 in every group and 6 in every time point. ① Activity of myocardium SOD： It was lower in the adrenaline group, adrenaline＋esmolol group at every time point than that in the sham operation group [At 30, 120 and 180 minutes after resuscitation： Those in the adrenaline group were （104.06±6.11 ）, （84.23±5.53）, （69.08±8.76）NU/mg; those in the adrenaline ＋esmolol group were （116.33 ±4.85）, （99.33 ±7.93）, （79.67 ±3.82）NU/mg; those in the sham operation group were （138.19±7.97）, （ 135.48±6.43 ）, （ 130.99±4.8.5 ）NU/mg,P 〈 0.01], and those were higher in the adrenaline＋esmolol group than that in the adrenaline group （P 〈 0.05）. ② The contents of myocardium malondialdehyde： It was higher in the adrenaline group and the adrenaline＋esmolol group at every time point than that in the sham operation group [At 30, 120 and 180 minutes after resuscitation in every group： Those in the adrenaline group were （1.5.97 ±0.99）, （21.48 ±2.84）, （33.90±4.39）μmol/g, those in the adrenaline ＋esmolol group were （12.88±1.12）, （16.04±1.75）, （23.59±.85）μmol/g; those in the sham operation group were （10.80±1.43）, （12.08±0.76）, （12.22 ±.84）μmol/g,P 〈 0.01], and it was lower in the adrenaline ＋esmolol group than that in the sham operation group （P 〈 0.01）. CONCLUSION： The esmolol can improve the oxygen-derived free radicals metabolism disturbance by means of alleviating the decrease of activity of SOD and the increase of content of malondialdehyde in postresuscitation myocardium.