佛甲草对小鼠心、脑缺氧的保护作用

Protective effects of sedum lineare thunb against cardiac and cerebral anoxia in mice

目的:观察佛甲草提取液对常压耐缺氧法、特异性心肌缺氧法、亚硝酸钠中毒法、脑缺血缺氧4种模型小鼠缺氧耐受性的影响。方法:实验于2004-03/06在赣南医学院机能实验室完成。选择昆明种小鼠170只,制备4种小鼠缺氧模型。①常压耐缺氧:取小鼠40只随机分成4组:生理盐水、盐酸普萘洛尔0.02g/kg组、佛甲草提取液10g/kg,5g/kg组,每组10只。腹腔注射给药后20min,将小鼠分别置于容积为250mL放有钠石灰的磨口广口瓶内,密封,观察小鼠存活时间。②特异性心肌缺氧:取小鼠50只随机分成5组,每组10只。生理盐水组、生理盐水+异丙肾上腺素组、盐酸普萘洛尔0.02g/kg组、佛甲草提取液10g/kg,5g/kg组。腹腔注射给药后40min,除生理盐水组外,其余各组小鼠均皮下注射异丙肾上腺素0.015g/kg,10min后,将小鼠分别放入250mL装有钠石灰的磨口广口瓶中,密封,记录小鼠存活时间。③亚硝酸钠中毒:取小鼠40只随机分成4组:生理盐水组、盐酸普萘洛尔0.02g/kg组、佛甲草提取液10g/kg,5g/kg组,每组10只。腹腔注射给药后40min,各组小鼠分别腹腔注射亚硝酸钠200mg/kg,记录小鼠存活时间。④脑缺血缺氧:取小鼠40只随机分成4组:生理盐水组小鼠、盐酸普萘洛尔0.02g/kg组、佛甲草提取液10g/kg,5g/kg组,每组10只。腹腔注射给药后40min,对各组小鼠在不麻醉的情况下快速断头,记录断头至最后一次喘息所需的时间。结果:170只小鼠均进入结果分析。①常压缺氧小鼠存活时间:生理盐水组存活时间(47.60±5.62)min,佛甲草提取液10g/kg,5g/kg组、盐酸普萘洛尔0.02g/kg组均能明显延长小鼠的存活时间犤(57.90±7.28)min,(53.90±7.29)min,(65.80±8.94)min,(t=2.164,3.542,5.451,P<0.05,0.01)犦。②特异性心肌缺氧小鼠存活时间:生理盐水组存活时间(47.60±5.62)min,生理盐水+异丙肾上腺素组显著缩短小鼠的存活时间(31.90±9.70)min,(t=4.423,P<0.001),佛甲草提取液10g/kg,5g/kg+异丙肾上腺素组、盐酸普萘洛尔0.02g/kg+异丙肾上腺素组均能明显延长小鼠的存活时间犤(46.80±9.70)min,(44.10±9.99)min,(51.10±10.93)min,(t=3.433,2.773,4.156,P<0.05～0.01)犦。③亚硝酸钠中毒小鼠存活时间:生理盐水组存活时间(17.00±2.94)min,佛甲草提取液10g/kg,5g/kg组、盐酸普萘洛尔0.02g/kg组均能非常显著的延长小鼠的存活时间犤(26.90±3.54)min,(23.60±3.98)min,(39.10±9.05)min,(t=4.218～7.345,,P<0.001)犦。④脑缺血缺氧小鼠存活时间:生理盐水组存活时间(19.70±3.56)min,佛甲草提取液10g/kg,5g/kg组、盐酸普萘洛尔0.02g/kg组均能明显延长小鼠的存活时间犤(26.40±4.93)min,(23.10±3.28)min,(30.30±5.38)min,(t=3.490,2.222,5.196,P<0.05～0.001)犦。结论:佛甲草提取液能延长小鼠在常压缺氧,特异性心肌缺氧、亚硝酸钠中毒性缺氧及脑缺血缺氧条件下的存活时间,显著提高缺氧小鼠的耐受性。

AIM： To observe effects of the solution isolated from sedum lineare thuMb on oxygen-deficient endurance in mice in 4 kinds of anoxia models including the test of the tolerance to anoxia under normal pressures, the test of the specificity myocardium under hypoxia, the test of the toxicity of sodium nitrite, and the cerebral ischemia-hypoxia test. METHODS： The experiment was conducted at Department of Organic Experiment, Gannan Medical College from March to June 2004. A total of 170 Kunming mice were selected and used it, 4 kinds of anoxia models, ① Hypoxia endurance at normal pressure： Totally 40 mice were randomly selected and divided into 4 groups： saline group, propranolole hydrochloride 0.02 g/kg group, extracts of sedum lineare thuMb 10 g/kg, 5 g/kg group with 10 mice in each group. Twenty minutes after the administration of the extract by intraperitoneal injection, the mice were put into closed hypoxic wide mouthed bottles of 250 mL volume with sodalime to observe the survival time of mice. ② Specific anoxic myocardium： Totally 50 mice were randomly selected and divided into 5 groups with 10 mice in each group： saline group, saline＋ isoproterenol group, 0.02 g/kg hydrochloric propranolol group and 10 g/kg, 5 g/kg extracts of sedum lineare thumb group. Forty minutes after the administration of the extract by intraperitoneal injection, except the saline group, the mice in other groups were all treated with subcutaneous injection of 0.015g/kg isoprotereno. Then 10 minutes later, the mice were put into closed hypoxic wide mouthed bottles of 250 mL volume with sodalime to observe the survival time of mice. ③ Antagonism of sodium nitrite： Forty mice were randomly divided into 4 groups with 10 mice in each group： saline group, 0.02 g/kg hydrochloric propranolol group and 10 g/kg,5 g/kg extracts of sedum lin-eare thunb group. Forty minutes after the administration of the extract by intraperitoneal injection, the mice were treated with 200 mg/kg sodium ni-trite by intraperitoneal injection, and the survival time was recorded. ③ The Cerebral ischemia-hypoxia： Forty mice were randomly divided into 4 groups with 10 mice in each group： saline group, 0.02 g/kg hydrochloric propranolol group and 10 g/kg,5 g/kg extracts of sedum lineare thunb group. Forty minutes after the administration of the extract by intraperitoneal injection, the heads of the mice were cut under the non-anesthesia and the gasping duration data of each mouse after decapitation was recorded. RESULTS： 170 mice were all involved in the result analysis. ① The survival time of mice under hypoxia at normal pressure： The survival time of the mice in the saline group was （47.60±5.62） minutes, and the survival time of the mice in the 10g/kg, 5 g/kg extracts of sedum lineare thunb group and 0.02g/kg hydrochloric propranolol group could be prolonged significantly [（57.90±7.28）, （53.90±7.29）, （65.80±8.94） minutes, （t=2.164, 3,542, 5.451, P 〈 0.05, 0.01 ）]. ② The survival time of mice with specific anoxic myocardium： The survival time of the mice in the saline group was （47.60±5.62） minutes; The survival time of the mice in the saline ＋ isoproterenol group could be shortened significantly （31.90±9.70）minutes,（t=4.423, P 〈 0.001 ）; The survival times of the mice in the 10 g/kg, 5 g/kg extracts of sedum lineare thunb ＋ isoproterenol group and the 0.02 g/kg hydrochloric propranolol ＋ isoproterenol group could be prolonged significantly [（46.80±9.70）, （44.10±9.99）, （51.10±10.93） minutes, （t=3.433, 2.773, 4.156, P 〈 0.05-0.01）]. ③ The survival time of mice under the antagonism of sodium nitrite： The survival time of the mice in the saline group was （17.00±2.94）minutes; The survival times of the mice in the 10 g/kg, 5 g/kg extracts.of sedum lineare thunb group and the 0.02 g/kg hydrochloric propranolol group could be prolonged significantly [（26.90±3.54）, （23.60±3.98）, （39.10±9.05）minutes, （t=4.218-7.345, P 〈 0.001 ）]. ④ The survival times of the mice with cerebral ischemia-hypoxia： The survival times of the mice in the saline group was （19.70±3.56）minutes; The survival times of the mice in the 10 g/kg, 5 g/kg extracts of sedum lineare thunb group and the 0.02 g/kg hydrochloric propranolol group could be prolonged significantly [（26.40±4.93）, （23.10±3.28）, （30.30±5.38）minutes, （t=3.490, 2.222, 5.196, P 〈 0.05-0.001 ）]. CONCLUSION： The extracts of sedum lineare thumb can prolong the duration under the condition of hypoxia at normal pressure, specific anoxic myocardium, the hypoxia of toxicities of sodium nitrite and the cerebral ischemia-hypoxia in mice, and increase significantly the endurance of the hypoxia mice.