蛋白质组技术进展及其在心血管研究中的应用

Advances in proteomic techniques and the application in cardiovascular research

目的:对蛋白质组学技术方面的方法学进展及其在心血管疾病研究中的应用作系统总结。资料来源:检索Medline1975-01/2005-04与蛋白质组技术在心血管疾病研究中应用相关的文章,检索词为“proteomics,cardiovasculardis-ease”,并限定文章语言种类为英文。同时检索中国期刊全文数据库1994-01/2005-02与蛋白质组技术在心血管疾病研究中应用相关的文章,检索词为“蛋白质组、心血管”,限定文章语言种类为中文。排除重复性研究和综述类文章。资料选择:对资料进行初审,选取包括蛋白质组技术进展和/或心血管疾病蛋白质组研究方向的相关文献,筛除明显不随机或无对照组的试验研究,对剩余文献查找全文。排除标准:重复性研究。资料提炼:收集到81篇关于蛋白质组技术在心血管疾病研究的文章,共收集到26篇符合纳入标准的文献。排除的55篇为重复性研究。资料综合:对基因组和蛋白质组关系、蛋白质样品制备、双向电泳、图像分析、质谱鉴定和生物信息学技术的进展,以及蛋白质组技术在心血管疾病研究中的应用进行综合整述。扩张性心脏病是利用蛋白质组学技术研究的最为深入的一类心血管疾病。蛋白质组学为探明缺血性心脏病的发病机制提供了良好的技术手段。蛋白质组技术的不断完善和发展必将为医学和相关领域的深入研究提供强有力的技术支持。结论:尽管目前蛋白质组学尚处于起步阶段,但是蛋白质组学以其深入揭示疾病状态下机体内所有蛋白质表达规律的优势在阐明人类心脏疾病的细胞和分子机制方面表现出极大的潜力。不久的将来,随着蛋白质组研究技术日趋完善,现行的结构蛋白质组学必然向功能蛋白质组学过渡,为心血管疾病的早期诊断、新药研发以及心脏康复开辟新的思路。

OBJECTIVE： To systematically review the methodological progress in proteomic techniques as well as their application in cardiovascular researches. DATA SOURCES： A computer-based search of the Medline database for relevant studies published in English between January 1975 and April 2005 was performed by using the keywords of “proteomics, cardiovascular disease”. Meanwhile, Chinese journals full-text database was scanned with computer to search relevant articles published in Chinese from January 1994 to February 2005, and the keywords were “proteomics, cardiovascular”. The repetitive studies and reviews were excluded. STUDY SELECTION： Literatures about advances in proteomic techniques and/or their application in cardiovascular researches were selected in the first searching, the obviously non-randomized ones and those without control group were screened, and then the full texts of the rest articles were looked up, the repetitive studies were excluded. DATA EXTRACTION： Totally 81 literatures about the application of proteomic techniques in cardiovascular studies were collected, and 26 of them were in accordance with the inclusive criteria, the excluded 55 articles were repetitive studies. DATA SYNTHESIS： The relationship between genome and proteome, progress in protein sample preparation, two-dimensional gel electrophoresis, image analysis, mass spectrometry and bioinformatics technology, as well as proteomic techniques in the application of cardiovascular study were reviewed respectively. Dilated cardiomyopathy is a kind of cardiovascular disease that has used proteomic techniques the most deeply. Proteomic techniques have provided good technical means for the exploration of the pathogenesis of ischemic heart disease. The constant perfection and development of proteomic techniques will provide a powerful technical support for the further study on medicine and relevant fields. CONCLUSION： Proteomics is still at the primary phase, but it manifests great potential in clarifying the cellular and molecular mechanisms of cardiac diseases by its advantages in deeply disclosing the in vivo expressive regularities of all proteins under the state of disease. In the near future, with the gradual perfection of proteomic techniques, there will be a newlyestablished idea on the early diagnosis, research and development of new drugs, as well as cardiac rehabilitation in connection with cardiovascular diseases, which results from functional proteomic study.