柳珊瑚酸及其衍生物对小鼠学习和记忆的影响

Effects of suberogorgin and its derivates on learning and memory in mice

目的：研究柳珊瑚酸（Ｓｕｂ）、Ｎ柳珊瑚酰胺ＮＮ二环己基脲（ＳｕｂＤＵ），Ｎ环己基柳珊瑚酰胺（ＮＣＳ）在影响记忆与抗胆碱酯酶（ＡＣｈＥ）作用间的关系．方法：在被动回避操作装置上测定小鼠ｉｐＳｕｂ或其衍生物后的跳台潜伏期（ＳＤＬ）和逃避潜伏期（ＥＬ）；比色法测定ＡＣｈＥ活力．结果：Ｓｕｂ１９，ＳｕｂＤＵ３０和Ｐｈｙｓ０１５ｍｇ·ｋｇ－１分别使成年小鼠的ＳＤＬ延长１９５％，２７１％和２１０％，ＥＬ缩短５６％，６１％和３３％；前二者尚同时使小鼠全脑匀浆ＡＣｈＥ活力各降低１７％和１９％．大于上述剂量时，Ｓｕｂ和ＳｕｂＤＵ对ＳＤＬ和ＥＬ的作用依剂量地减弱．Ｓｕｂ１９，ＳｕｂＤＵ２０和Ｐｈｙｓ０１５ｍｇ·ｋｇ－１还可使老年小鼠的ＳＤＬ各延长１８７％，２０９％和１５２％，ＥＬ各缩短５２％，６２％和５７％．三药尚明显改善由环己酰亚胺或东莨菪碱产生的记忆保持损害．ＮＣＳ无上述作用．结论：Ｓｕｂ和ＳｕｂＤＵ对记忆的改善作用与其抗ＡＣｈＥ有关．

AIM: To study the relationship between the effects of suberogorgin (Sub) and its derivates on memory and their anti acetylcholinesterase (AChE) actions. METHODS: The step down latency (SDL) and the escape latency (EL) of mice were determined at the same time in a passive avoidance task after Sub, N subero￣gorgamide N N dicyclohexyl urea (Sub DU), or N cyclohexyl suberogorgamide ( N CS) was injected ip. The AChE activities in brain hemogenates were determined with colorimetry. RESULTS: Sub 1 9, Sub DU 3 0, or physostig￣mine (Phys) 0 15 mg·kg 1 obviously lengthened the SDL by 195 %, 271 %, and 210 %, and shortened the EL by 56 %, 61 %, and 33 %, and the two formers inhibited the brain AChE activities by 17 % and 19 %, respectively in aging (3-4 months) mice. These actions were decreased in a dose dependent manner when Sub or Sub DU was increased to 2 9-4 3 or 4 5-6 7 mg·kg 1 respectively. Sub 1 9, Sub DU 2 0, and Phys 0 15 mg·kg 1 also lengthened the SDL by 187 %, 209 %, and 152 %, and shortened the EL by 52 %, 62 %, and 57 %, respectively in aged (12-14 months) mice. Sub 1 3-1 9, Sub DU 0 9-2 0, or Phys 0 15 mg·kg 1 reversed the cycloheximide or scopolamine induced disruptions of memory retention. No obvious effect of N CS on the acquisition of memory and the AChE activity in mice was observed. CONCLUSION: The improvements of Sub and Sub DU on memory were chiefly related to their anti AChE actions.