槲皮素对肿瘤坏死因子和白细胞介素－1β前破骨活性的抑制作用

Inhibitory effect of quercetin on tumor necrosis factor and interleukin 1β

目的：测试槲皮素对肿瘤坏死因子（ＴＮＦ）和白细胞介素１β（ＩＬ１β）两种前破骨活性的影响．方法：［３Ｈ］ＴｄＲ参入法观察细胞增殖．以对硝基苯酚磷酸酯为底物用微量分光光度法测定细胞内碱性磷酸酶活性．放射免疫法测定前列腺素Ｅ２（ＰＧＥ２）．结果：槲皮素（５－４０μｍｏｌ·Ｌ－１）以浓度依赖方式减少ＴＮＦ和ＩＬ１β对成骨细胞增殖和碱性磷酸酶活性的抑制作用，２０－４０μｍｏｌ·Ｌ－１时减少两种细胞因子刺激成骨细胞产生ＰＧＥ２．结论：槲皮素对ＴＮＦ和ＩＬ１β两种前破骨活性有抑制作用．

IM: To study the effects of quercetin on tumor necrosis factor (TNF) and interleukin 1β (IL 1β) pro osteoclastic activities. METHODS: TdR uptake by osteoblasts was used to measure cell proliferation, microspectrophotometer for cellular AlP activity using p nitrophenyl phosphate as enzyme substrate, and radioimmunoassay for prostaglandin E 2. RESULTS: Quercetin 5-40 μ mol·L -1 reduced the inhibition of cell proliferation and AlP activity induced by TNF or IL 1β in a concentration dependent manner. PGE 2 produc￣tion stimulated by either cytokines was also reduced by quercetin at 20 and 40 μmol·L -1 . CONCLUSION: quercetin exerted a marked inhibitory effect on TNF and IL 1 activities, related to their pro osteoclastic function.