广防己致大鼠慢性肾小管-间质损伤的实验研究Ⅱ

Experimental study of chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi extract in rats

目的:接前报继续观察广防己提取物诱导大鼠慢性肾损害作用,进一步了解不同剂量的广防己提取物(RAFE)可否引起慢性肾小管-间质损伤。方法:分别用广防己醇提取物25.0,120.0,200.0 mg.kg-1.d-1和马兜铃总酸10.0 mg.kg-1.d-1给大鼠间断(连续2周,停药1周)灌胃给药22周后,停药4周;分别于给药第17,22,26周留取血、尿和肾组织标本,检测相关肾功能指标;并取肾脏进行形态组织学观察,于给药第17,22,26周对肾组织进行Masson染色,并与马兜铃总酸进行比较。结果:RAFE作用肾组织形态学病变:小、中、大剂量和总酸组主要表现为肾小管功能损害———慢性间质纤维化,这些病理表现与马兜铃总酸所致肾损害极为相似。结论:RAFE小、中、大剂量灌胃给药13周以上可致大鼠慢性肾小管-间质纤维化,其肾功能和肾组织形态表现上与马兜铃总酸所致肾损伤相似,结果提示马兜铃酸是RAFE主要毒性成分。

Objective： Following the former report, we continue to observe the chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi Extract（RAFE） in rats in order to understand whether RAFE in different doses causes the renal tubular-interstitial injury or not. Method： RAFE at the dose of 25.0mg·kg^-1·d^-1, 120.0mg·kg^-1·d^-1 and 200.0mg·kg^-1·d^-1 and aristolochic acid （AA, 10. 0mg·kg^-1·d^-1） was interruptedly administrated by gastric tube for 22 w and 4 w durg withdrawal. Blood, urine and kidney were taken out respectively in 17 w, 22 w and 26 w to measure the indexes of renal function. The morphology of kidney was observed, and Masson staining of kidney were made respectively to compare RAFE groups with AA group. Result： Pathological changes of renal tissue forms were as follows： All RAFE groups and AA group could develop the pathological process of renal tubular injury-chronic renal interstitial fibrosis. The pathologic changes of RAFE were similar with AA. Conclusion： RAFE at all doses administrated interruptedly by gastric tube above 13 w caused chronic renal tubulo-intersfitium fibrosis. The renal injury in functions and tissue forms in rats were similar with AA closely. The results showed that AA was the main toxic composition of RAFE.