慢性氟中毒大鼠垂体生长激素细胞及下丘脑生长抑素神经元的免疫细胞化学研究

IMMUNOCYTOCHEMICAL STUDIES ON GROWTH HORMONE(GH) CELLS OF PITUITARY AND SOMATOSTATIN(SS) NEURONS OF HYPOTHALAMUS IN EXPERIMENTAL CHRONIC FLUOROSIS RATS

本实验对慢性氟中毒大鼠垂体生长激素(GH)细胞及下丘脑生长抑素(SS)神经元进行了免疫细胞化学染色,并用图像分析仪对GH及SS进行了半定量分析。结果表明:同对照组相比,氟中毒垂体GH细胞中的阳性反应颗粒细小并弥散地分布,半定量分析表明其GH含量明显低于对照组。未见下丘脑SS神经元及其SS含量有明显改变。本研究结果提示高氟可直接作用于垂体GH细胞影响其合成分泌功能。

The male rats model of chronic fluorosis was established with administration of drinking water containing 100mg/L fluoride (as NaF). The GH cells in pituitary and SS neurons in hy-pothalamus were stained with immunocytochemical method. The result revealed the decrease in GH content in pituitary but no change of SS content in hypothalamus with fluorosis significantly- This result suggested that administration of large dose of fluoride caused the damage of GH cells directly and affects the synthesis and secretion of GH.