摘要
用甲醇回流浸提苦瓜总甙,并经硅胶柱分离制备单体皂甙MomordicosideA。以MomordicosideA为标样,选择波长546nm,采用分光光度法,建立了苦瓜总皂甙含量测定方法。实验结果表明:全苦瓜干粉中总皂甙质量分数为0.0432%;标准曲线下,皂甙含量自40μg到200μg呈线形关系,回归方程为A=0.0058C-0.1125(R2=0.9994);加样回收率为102.72%,RSD为3.99%。本法操作简便、快速、准确,可作为苦瓜原料及苦瓜制品质量评价方法。
A method for the determination on total saponins of Momordica charantia L. was established： using MeOH by continuous thermal reflux to extract its total saponins, taking mordicoside A as standard sample, selecting 546nm wavelength and by spectrometric method. The results showed that the Total Saponins accounted for 0.0432% of the dried powder of Momordica charantia L. And the regression equation within the range of the standard sample concentration in 40～200 μ g was （A=0.0058C - 0.1125） with a good linearity （R^2=0.9994）.The average recovery rate was 102.72% and RSD =3.99%.The method is simple, rapid and accurate and suitable for the quality evaluation of Momordica Charantia L. material and products.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第10期165-169,共5页
Food Science
基金
江苏省科技攻关项目(BE2004360)
关键词
苦瓜
总皂甙
苦瓜甙A
分光光度法
测定
Momordica charantia L
total saponins
Mordicoside A
spectrometric method
determination
