大豆异黄酮抗氧化效能的研究

Studies of Soy Isoflavones on Antioxidation

目的:观察大豆异黄酮对小鼠抗氧化效能的影响。方法:选用BALB/C雄性小鼠60只,体重18～22g,随机分为4组:空白对照组、低剂量组、中剂量组和高剂量组,空白对照组喂饲基础饲料,其余3组分别喂饲添加25、100、200mg/kg大豆异黄酮的基础饲料。实验第8周末,将小鼠断头处死,取血、心脏、肝脏组织测定SOD、GSH-Px、CAT、MDA、H2O2和T-AOC等指标。结果:与空白对照组比较,小鼠肝脏中的MDA和H2O2含量显著降低(p<0.01),小鼠全血和肝脏、心脏GSH-Px和CAT活性明显升高(p<0.01),肝脏、心脏SOD活性,T-AOC含量明显升高(p<0.01)。结论:大豆异黄酮具有明显的抗氧化作用。

Objective： To investigate the antioxidation effects of soy isoflavones in mice during an experimental period of 8 weeks. Methods： 60 BALB/C male mice （18～22g, BW） were divided randomly into 4 groups and fed a basal diet supplemented with 25,100, 200mg/kg soy isoflavones. Results：The MDA and H2O2 levels in liver were significantly lower than those of the control group （p 〈 0.01）. The GSH-Px and CAT activities of in liver and whole blood were increased obviously （p 〈 0.01）. The SOD, T-AOC levels in liver were significantly increased （p 〈 0.01）. Conclusion： These results suggested that soy isoflavones can improve the ability of antioxidation obviously.