BMP-4和Ki-67在泌乳素腺瘤中的表达及临床意义

Expressions of BMP-4 and Ki-67 protein in prolactinomas and their clinical significance

目的探讨骨形态生成蛋白4(BMP-4)和细胞核增殖相关抗原(Ki-67)在泌乳素(PRL)腺瘤中的表达及与腺瘤侵袭性的关系.方法应用免疫组织化学和原位杂交技术检测 35例PRL腺瘤及8例正常垂体组织中BMP-4mRNA、BMP-4和Ki-67蛋白的表达水平,并对三者的表达水平的关系进行统计分析.结果 8例正常垂体组织的BMP-4和Ki-67表达均阴性;35例PRL腺瘤中,BMP-4mRNA、BMP-4表达水平与PRL腺瘤的侵袭性呈正相关 (r=0.885,P＜0.01);Ki-67在2～3级与0～1级的表达水平有显著性差异(P＜0.05),但 BMP-4mRNA、BMP-4的表达较Ki-67更具灵敏性(P＜0.05).结论 BMP-4在PRL腺瘤中呈特异性高表达,且与PRL腺瘤侵袭性呈正相关.

Objective： To investigate the expressions of bone morphogenetic protein-4（BMP-4） and Ki-67 in prolactinomas and their relationships with invasiveness characteristics. Methods： The expressions of BMP- 4mRNA,BMP-4 and Ki-67 protein in 35 cases of prolactinomas and 8 cases of normal pituitary tissues samples were detected by immunohistochemical and in situ hybridization technique,and the results were analysised by statistic method and their relationships were found. Results.. The expressions of BMP-4mRNA, BMP-4 and Ki- 67 was negative in normal pituitary tissues,and their expressions in 35 cases of prolactinomas was positively related with invasiveness grades（ P 〈0.01）. The expressions of Ki-67 protein in grades 2-3 were significantly different from those in grades 0-1 in prolactinomas （P 〈0.05）. So the expressions of BMP-4mRNA, BMP-4 were more sensitive than the Ki-67 （P 〈 0.05 ）, Conclusions： The BMP-4 was highly expressed in the prolactinomas and was positively related with the invasiveness grades.