甘蓝型油菜白花基因的RAPD标记

RAPD markers linked to white-petal gene in Brassica napus L.

以甘蓝型白花油菜及其分离群体为材料,对甘蓝型油菜白花性状的遗传规律进行了研究。结果表明,白花对黄花是一对由不完全显性基因(WW)控制的不完全显性遗传性状;利用集群分离法(BSA)对该白花基因进行RAPD分析,在1 200个随机引物中,获得了引物S1092(5-CCC AGG CTA C-3),在白花基因库和黄花基因库间扩增出多态性产物S-10921250;对该分离群体及其姊妹系和其他白花油菜进行的单株验证表明,S-10921250与甘蓝型油菜白花基因相连锁,遗传距离为0.84 cM。

White-petal （W） rapeseed （Brassica napus L. ） and its segregation population were used as experiment materials to study the inheritance of its white-petal traits. The result showed that the petal color character of W vs Y （yellow-petal） was controlled by one pair incomplete dominant gene WW. Bulked segregation analysis （BSA） was employed to identify random amplified polymorphic DNA （RAPD） markers linked to the white-petal gene. A total of 1 200 arbitrary 10-met oligonucleotide primers were screened on the DNA of W and Y bulks. Primer S1092（5-CCC AGG CTA C-3） gave polymorphism between the two bulks. DNA from Individual plants of the two bulks,their sister lines and the other white-petal rapeseed （Brassica napus L. ） were then used as template for screening with the primer S1092 and the 1.25 kb DNA polymorphism generated by the primer S1092 were verified linking with the white-petal gene. The genetic distance was 0.84 cM between RAPD marker and white-petal gene.