摘要
以甘蓝型白花油菜及其分离群体为材料,对甘蓝型油菜白花性状的遗传规律进行了研究。结果表明,白花对黄花是一对由不完全显性基因(WW)控制的不完全显性遗传性状;利用集群分离法(BSA)对该白花基因进行RAPD分析,在1 200个随机引物中,获得了引物S1092(5-CCC AGG CTA C-3),在白花基因库和黄花基因库间扩增出多态性产物S-10921250;对该分离群体及其姊妹系和其他白花油菜进行的单株验证表明,S-10921250与甘蓝型油菜白花基因相连锁,遗传距离为0.84 cM。
White-petal (W) rapeseed (Brassica napus L. ) and its segregation population were used as experiment materials to study the inheritance of its white-petal traits. The result showed that the petal color character of W vs Y (yellow-petal) was controlled by one pair incomplete dominant gene WW. Bulked segregation analysis (BSA) was employed to identify random amplified polymorphic DNA (RAPD) markers linked to the white-petal gene. A total of 1 200 arbitrary 10-met oligonucleotide primers were screened on the DNA of W and Y bulks. Primer S1092(5-CCC AGG CTA C-3) gave polymorphism between the two bulks. DNA from Individual plants of the two bulks,their sister lines and the other white-petal rapeseed (Brassica napus L. ) were then used as template for screening with the primer S1092 and the 1.25 kb DNA polymorphism generated by the primer S1092 were verified linking with the white-petal gene. The genetic distance was 0.84 cM between RAPD marker and white-petal gene.
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2005年第10期57-61,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
陕西省自然科学研究计划项目[2001SM32]