高效液相色谱法同时测定胃痛舒胶囊中原阿片碱和白屈菜碱含量

Content Determination of Chelionine and Protopine in Wei Tong Shu Capsule

目的:建立胃痛舒胶囊中有效成分中原阿片碱和白屈菜碱含量的高效液相色谱测定法.方法:以Shimpack ODS柱(4.6 mm×250 mm,5 μm)为分析柱:甲醇-1%三乙胺溶液(65∶35)为流动相;285 nm检测,建立了胃痛舒胶囊中原阿片碱和白屈菜碱含量测定的HPLC-UV法.结果:所建立的含量测定方法中原阿片碱在进样量0.084 8～0.424 0 μg范围内呈良好的线性关系(r=0.999 6):日内和日间RSD分别为1.62%和1.89%:平均加样回收率为98.38%.白屈菜碱在进样量0.208 0～ 1.040 μg范围内呈良好的线性关系(r=0.999 7);日内和日间RSD分别为1.49%和2.04%;平均加样回收率为99.23%.结论:以上方法简便、准确、重现性好,可用于胃痛舒胶囊中原阿片碱和白屈菜碱含量测定.

Objective：To establish an HPLC method for the determination of chelinine and protapine in Wei Tong Shu capsule. Methods： ODS column as analytic column ; methanol-1% triethylamine （65：35） as mobile phase,285nm as detective wavelength to establish the HPLC - UV method. Results： The established HPLC-UV method had good linearity and precision within the range of 0.0848-0.4240μg of protopine and 0.2080-1. 040μg of chelionine,the average recovery of protopine and chelionine was 98.38% and 99.23%, respectively. Conclusion：The method is simple, accurate and precise for the determination of chelionine and protopine in Wei Tong Shu Capaule.