黄芪提取成分对冲击波致家兔肾损伤模型中P-选择素表达的影响

Effects of components isolated from Astragalus mongholicus on expression of p-selectin in shock wave induced kidney injury in rabbit model

目的:探讨黄芪抗体外冲击波碎石术(ESWL)致肾损伤的有效成分及作用机制。方法:69只家兔随机分为假处理组、对照组、黄芪总皂苷组、总黄酮组和总多糖组,观察ESWL前后P-选择素(p-selectin)在肾脏的表达及细胞超微结构的改变,测定肾组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果:ESWL后p-selectin广泛表达于肾小球、肾小管及肾间质等部位,肾组织MDA含量升高,SOD活性明显下降(P<0.05)。黄芪总皂苷组p-se-lectin阳性率及细胞损伤程度显著降低(P<0.05),MDA含量及SOD活性分别明显低于和高于对照组水平(P<0.05)。黄芪总黄酮与总多糖对p-selectin无抑制作用,二者抗冲击波致肾损伤的能力均不如总皂苷显著。结论:黄芪总皂苷除具有清除自由基的能力外,还可显著抑制黏附分子p-selectin的异常表达,是黄芪抗冲击波致肾损伤的主要效应成分。

Objective： To investigate the effective components of Astragalus monghokucys and their mechanisms in alleviating extracorporeal shock wave lithotripsy （ESWL） induced kidney injury. Method： 69 male rabbits were randomly assigned into control group, sham treatment group, Total Saponins of Astragalus （TSA） group, Total FLavonoids of Astragalus （TFA） group, and Total Polysaccharide of Astragalus group （TPA）. The shock wave treated kidneys were observed for the expression of p-selectin and the change of cells ultrastmcture pre and post ESWL. The concentration of Maleic Dialdehyde （MDA） as well as activity of superoxide dismutase （SOD） in the kidney tissues was also analyzed. Result： After application of shock wave treatment, p-selectin was expressed extensively in renal glomerulus, renal tubules and renal interstitium of the treated kidneys. It showed a significant increase of MDA levels and decrease of SOD activity in control group （ P 〈 0.05） after ESWL. The comparison between controls and TSA group demonstrated that TSA could significantly reduce the positive rate of p-selectin （ P 〈 0. 05） and alleviate the injuries of cells ultrastructure. The results also showed a significant decrease of MDA levels and increase of SOD activity in TSA group compared to controls （P 〈 0.05）. The protective effects of TFA and TPA in alleviating kidney injury induced by shock wave were lower than those of TSA; they had no effects of the expression of P-selectin. Conclusion： TSA is the main components of A. mongholicus in alleviating shock wave induced kidney injury not only by scavenging oxygen free radicals but also inhibiting the expression of p-selectin.