清热化湿方对脾胃湿热证患者胃黏膜AQP3、AQP4蛋白表达的影响

Effects of Qingre Huashi Prescription on AQP3 and AQP4 Expressions in Gastric Mucosa of the Patient with Damp-heat in the Spleen and the Stomach

目的:探讨脾胃湿热证与胃黏膜水通道蛋白(AQP3)、AQP4表达的关系俄语及中药清热化湿方的作用机制。方法:选择慢性浅表性胃炎患者68例,其中脾胃湿热证53例(湿重于热19例,热重于湿14例,湿热并重20例),脾虚证15例;另选正常健康体检者10例为正常对照组。脾胃湿热证患者予中药清热化湿方,并采用免疫组化方法检测入选者胃黏膜 AQP3、AQP4表达情况。结果:AQP3、AQP4在脾胃湿热证组的表达明显强于正常对照组(P<0.05);脾虚证组与正常组比较差异无显著性(P>0.05);各亚型之间呈湿重于热>湿热并重>热重于湿的趋势,但差异无显著性(P>0.05)。治疗后,脾胃湿热证组 AQP3、AQP4表达水平显著下降(P<0.01),与正常对照组及脾虚证组比较差异无显著性(P>0.05)。结论:AQP3、AQP4的异常表达可能是脾胃湿热证的发生机制之一,中药清热化湿方可能通过影响 AQP3、AQP4的表达而发挥治疗作用。

Objective： To explore the relation of the spleen and stomach damp-heat syndrome with AQP （aquaporin）3 and AQP4 expressions, and the mechanism of the action of Qingre Huashi Prescription. Methods： 68 cases of chronic superficial gastritis, including 53 cases of spleen and stomach damp-heat syndrome （19 cases with more heavy dampness, 14 cases with more heavy heat, 20 cases with equal dampness and heat）, 15 cases with spleen deficiency syndrome, 10 cases with normal findings by gastroscopy used as normal group. The patients with spleen and stomach damp-heat syndrome were treated with Qingre Huashi Prescription, and AQP3 and AQP4 expressions were detected with immunohistochemical method. Results： AQP3 and AQP4 expressions in the spleen and stomach damp-heat group were significantly stronger than those in the normal group （P〈0. 05）. There was no significant difference between the spleen deficiency group and the normal group （P〉0.05）; and there was no significant difference among the subgroups （P〉0.05）. After treatment, the expressions of AQP3 and AQP4 in the spleen and stomach damp-heat syndrome group decreased significantly（P〈0. 01）, with no significant difference as compared with the normal group and the spleen deficiency group. Conclusion： Abnormal expression of AQP3 and AQP4 is possibly one of the mechanisms of spleen and stomach damp-heat syndrome generation, and Qingre Huashi Prescription exerts therapeutic effect possibly through influencing expression of AQP3 and AQP4.