金属硫蛋白及其基因在人胰腺癌细胞株中的表达及意义

Metallothionein and Its Isoform Genes Expression in the Human Pancreatic Cancer Cell Strains and Their Function

目的比较金属硫蛋白(MT)及其基因在人胰腺癌细胞株和耐药细胞株中的表达,探讨MT表达与胰腺癌细胞耐药的关系。方法采用逆转录聚合酶链反应(RT-PCR)检测MT基因的mRNA表达情况,采用镉/血红蛋白饱和-电化学分析法检测MT表达情况。结果人胰腺癌细胞株中的MT可由MT-1A、MT-1B、MT-1E、MT-1F、MT-1G、MT-1X及MT-2A基因编码;MT-1B、MT-1E、MT-1X及MT-2A基因和MT在人胰腺癌耐药细胞株中的表达水平显著增高(P<0.05)。结论人胰腺癌细胞株中的MT及其基因表达与胰腺癌细胞株的增殖及化疗耐药性相关。

Objective To compare the expression of metallothionein （MT） genes and proteins in six human pancreatic cancer cell strains and two human pancreatic cancer drug-resistant cell strains and to explore the relationship between the expression of the MT and pancreatic cancer cell chemoresistance. Methods Reverse transcriptase-polymerase chain reaction （RT-PCR） was used to determine the MT isoform-specific mRNA, and cadmium/hemoglobin saturation-electrochemistry to determine MT protein levels. Results MT protein expression in the pancreatic cancer cell strains was encoded by MT-1A, MT-1B, MT-1E, MT-1F, MT-1G, MT-1X, and MT-2A genes. The expression of MT proteins was upregulated and MT-1B, MT-1E, MT-1X, MT-2A genes overexpressed in human pancreatic cancer drug-resistant cell lines （P〈0.05）. Conclusion Expressions of MT proteins and genes correlate with the proliferation and chemoresistance of human pancreatic cancer cell strains.