同步荧光光谱法测定尿液中依诺沙星含量

Determination of Enoxacin in Urine by Synchronous Fluorimetry

文章研究了金属离子对依诺沙星荧光光谱的影响.实验结果表明在pH 6.3的NaH2PO4-Na2B4O7缓冲溶液介质中铝离子对依诺沙星荧光具有增敏作用,在此基础上用同步荧光技术建立了测定人体尿液中依诺沙星含量的同步荧光光谱法,方法简便快捷,线性范围为0.04～1.0 mg·L-1.相关系数为0.999 2.检出限为0.018μg·mL-1.在实际样品的测定当中,回收率在95%～105%之间.

The effect of metal ions on uhe fluorescence spectra of enoxacin was studied in detail. It has been found that aluminum can enhance the fluorescence intensity of enoxacin remarkably in the buffer solution of NaH2PO4-Na2B4O7 with pH 6.3. A new method using synchronous fluorimetry has been developed for the determination of enoxacin in human urine. The synchronous fluorescence intensity of Al/ENX system is linearly proportional to the concentration of ENX in the concentration range of 0.04-1.0μg·mL^-1, The detection limit, calculated according to IUPAC recommendations, was 0.018μg·mL^-1 . The precision of the method was established by repeated assays （n = 12） using 0.4μg·mL^-1 solution of ENX, and the relative standard deviation was 3.1%. The method was satisfactorily applied to the determination of enoxacin in human urine samples. The recovery was within the range of 95 %-105 %.