固体热解塞曼原子吸收光谱法用于中药和生物样品中痕量汞的快速测定

Determination of Mercury in Chinese Medicinal Material and Biological Samples Using Pyrolysis Atomic Absorption Spectrometry

建立了一种采用固体热解塞曼原子吸收光谱快速测定中药和生物样品中痕量汞的方法,样品不需要经过消解即可直接测定.考察了样品颗粒大小对分析结果的影响,在优化的实验条件下,方法的重现性为2.1%(峰面积)和9.1%(峰高),检出限为6.3 ng·g-1(3σ),回收率为95%～105%.使用标准参考物质进行对照分析,通过与ICP-MS分析法的结果进行比较,结果令人满意.大鼠灌胃实验表明,长期灌胃较大剂量的朱砂对肾等组织有一定的毒害,而在部分传统中药中朱砂用量较高.文章对制定中药安全性标准具有参考意义.

In this paper a rapid and simple method using pyrolysis coupled with atomic absorption spectrometry for the analysis of total mercury in Chinese medicinal material and biological samples is presented. No sample digestion was needed and this greatly simplifies the analytical procedure and minimizes potential sources of contamination. Under optimum conditions, the reproducibility of the method was 2.1% for peak area and 9.1% for peak height. The detection limit （3a） was 6.3 ng·g^-1 , and the recovery was within the range of 95 %-105 %. Several standard reference materials were analyzed and the results were obtained with satisfaction. The performance of the method was compared with inductively coupled plasma-mass spectrometry （ICP-MS）, and excellent agreements were observed between these two methods.