谷胱甘肽S-转移酶π、DNA拓扑异构酶Ⅱ及P-糖蛋白在难治性癫癎患者脑内的表达

Glutathione S-transferase π, DNA topoisomeraseⅡ, and P-glycoprotein expression in brain of patients with refractory epilepsy

目的探讨谷胱甘肽S-转移酶π(GST-π)、DNA拓扑异构酶Ⅱ(TopoⅡ)及P-糖蛋白(P-gp)在难治性癫癎(RE)患者脑内的表达及意义。方法32例RE患者手术切除标本,采用免疫组化方法检测GST-π、TopoⅡ及P-gp表达。8例脑血管畸形患者作为对照。结果对照组正常脑组织内星形胶质细胞及血管内皮细胞中有少量GST-π表达(2·2),癫癎组GST-π表达明显增强(10·9,P<0·05)。TopoⅡ在对照组中无表达,癫癎组2例标本有少量表达(0·4,P>0·05)。对照组中P-gp只在脑内血管内皮细胞中少量表达,癫癎组P-gp则在星形胶质细胞及血管内皮细胞中大量表达(12·9,P<0·01)。结论GST-π、P-gp在RE患者脑内表达可能与患者对抗癫癎药耐药有关。

Objective To investigate the effects and expression of glutathione S-transferase （GST-π）, DNA topoisomerase Ⅱ （Topo Ⅱ ） and P-glycoprotein （P-gp） in brain of patients with refractory epilepsy.Motheds The specimens were obtained from 32 patients with refractory epilepsy and 8 patients with arteriovenous malformations as controls, and immunohistochemistry for GST-π, Topo Ⅱ and P-gp was used. Results GST-π immunoreactivity in several capillary endothelial cells and astrocytes was found in normal brain samples （2. 2 ± 2. 6）, it was significantly increased in samples from epileptic patients （ 10. 9 ± 9. 8, P 〈 0. 05）. Topo Ⅱ immunoreactivity was not identified in normal samples, but found in 2 samples of epileptic patients （0. 4 ± 1.6, P 〉0. 05）. P-gp immunoreactivity was only found in capillary endothelial in normal samples, but it was identified in several astrocytes and increased in capillary endothelial cells in samples from epileptic patients （12.9 ±9.7, P〈0,01）. Conclusion Overexpression of GST-π, P-gp in patients with refractory epilepsy might play a role in pharmacoresistance to antiepileptic drugs,