毛白杨转rolB基因植株生根能力的研究

The rooting ability of rolB transformed clones of Populus tomentosa.

为了提高毛白杨的生根能力,把rolB基因转入毛白杨中,获得7个转化植株.在无激素培养基上,转化植株的生根率达到80%～100%,而未转化植株的生根率只有13%～20%.转化植株对生长素表现出高度的敏感性,当IBA浓度达1.0mgL时,转化植株的生根受到抑制.移栽后,1个转化株系的高生长明显低于对照,2个转化株系的茎节数比对照增加,3个转化株系的节间长比对照降低,其他转化植株与对照无明显差异.硬枝扦插试验结果进一步证实了转化植株扦插生根能力显著提高,扦插生根率达到74.4%～92.3%,根的数量也明显增加.

To improve the rooting ability of Populus tomentosa, the rolB gene was transformed into the plant and 7 transgenic clones were obtained. The in vitro rooting results showed that the rooting rate of transgenlc clones ranged from 80% to 100% without auxin, while only 13%-20% for the control plants. Transgenic plants showed a high sensitivity to the auxin. When the concentration of IBA reached 1.0 mg/L, the rooting of the transgenic clones was restrained. Growth analysis showed that the stem length of one transgenic clone was reduced and the node number of two clones was increased and the internode length of three clones was obviously reduced compared with the control plants. The hardwood cutting experiment further confirmed that the increased rooting ability of the transgenic clones ranged from 74.4% to 92.3%, and the root number was also greatly increased.