鼻咽癌相关基因BRD7对鼻咽癌细胞CNE1的影响

Study of NPC-related Gene BRD7 Effect on NPC Cell Line CNE1

为了研究BRD7基因对鼻咽癌细胞CNE1的影响,通过脂质体转染方法,将BRD7基因导入NPC细胞株CNE1细胞中.通过细胞生长曲线发现该基因能够抑制CNE1细胞的生长.为了探讨可能的作用机制,进而采用蛋白质组技术研究该基因对鼻咽癌蛋白质表达谱的影响,从而研究该基因在CNE1中的地位和作用.通过对过表达BRD7基因后鼻咽癌细胞系CNE1的蛋白质表达谱改变的研究,鉴定出19个差异表达蛋白,这些蛋白质包括:BCCIP(BRCA2andCDKN1A(p21(Waf1/Cip1)),FHL2(fourandahalfLIMdomains2),Chloridechannelregulatoryprotein;Hin-1(high-in-normal-1),WISP-1(connectivetissuegrowthfactorrelatedprotein),SREC-4(scav-engerreceptorexpressedbyendothelialcells-2),folatereceptor.这些差异蛋白涉及到基因表达调控、细胞黏附等众多的事件.从另一个侧面研究了BRD7基因与鼻咽癌的关系,扩展了BRD7基因的研究范围,并进一步充实了该基因做为鼻咽癌候选抑瘤基因的证据.

In oder to study effect ofBRD7 gene on NPC cell line CNE1, BRD7 was introduced into CNE1 cells by liposome transfection. BRD7 transfected cells were resulted in a declined growth curve. To account for the mechanism of this gene function on CNE1, two-dimensional polyacrylamide gel eletrophoresis（2-D PAGE） and MALDI-TOF were performed. After image analysis and MALDI-TOF identification, 19 differential expression proteins were identified. These proteins included BCCIP（BRCA2 and CDKN1A （p21 （Waf1/Cip1））, FHL2（four and a half LIM domains 2）, Chloride channel regulatory protein, Hin-1 （high-in-normal-1）, WISP-1 （connective tissue growth factor related protein）, SREC-4（scavenger receptor expressed by endothelial cells-2）, folate receptor, which involved in transcription regulation, adherence and so on. The study extended the research field of BRD7 and reinforced the evidences that BRD7 act as a NPC-related candidate suppressor gene.