改良协调性金黄地鼠到大鼠的异位腹腔异种心脏移植模型的建立

Establishment of hamster-to-rat cardiac concordant xenotransplantation model

目的:建立一种改良的异位腹部异种心脏移植模型,解决模型建立中的技术问题. 方法:利用袖套法和cuff套管法完成从金黄地鼠到大鼠的异种心脏移植模型. 将供心的颈总动脉与受者左肾动脉进行袖套式吻合,将供心右肺动脉与受者左肾静脉利用套管法吻合. 每日通过对受体进行左侧腹壁触诊完成对供心功能的评价. 结果:共实施105例异位腹部异种心脏移植模型,95例成功. 异种移植物生存期为移植后3～4 d,并且发生经典急性血管性排斥反应. 结论:建立了一种简便、实用、稳定的用于研究协调性异种移植免疫排斥反应发生机制的动物模型.

AIM： To establish a simplified method of heterotopic abdominal cardiac xenotransplantation. METHODS： The hamster to rat cardiac xenotransplantation was performed by means of “sleeve and cuff” method. The donor＇s common carotid artery was anastomosed to the recipient＇s left renal artery with a “sleeve” anastomosis, and the “cuffed” right pulmonary artery was anastomosed to the left renal vein. The viability of the donor＇s heart was examined by daily palpation of the left abdominal wall. RESULTS： Of the 10.5 cases receiving heterotopic abdominal cardiac xenotransplantation, complete success was achieved in 9.5 cases. The xenograft survived for 3 to 4 d after the transplantation and typical acute vascular rejection was presented. CONCLUSION： A useful, easy and stable model has been established for the investigation of the mechanism of the concordant xenotransDlantation.