鼻咽癌组织DNA聚合酶β基因突变与EBV感染相关

DNA polymerase β gene mutation in human nasopharyngeal cancer and its relationship with EBV infection

目的:研究探讨鼻咽癌中DNA聚合酶β(DNA pol-ym erase,βpolβ)的变异情况及与EBV感染有无相关关系.方法:采用RT-PCR法(逆转录-DNA聚合酶链反应)、PCR-SSCP(聚合酶链-单链构象多态性分析)、DNA序列分析法对26例鼻咽癌组织及癌旁组织标本进行polβ基因突变研究.用特异性EBV引物PCR扩增法检测EB病毒,分析出现EBV-PCR阳性与polβ突变及鼻咽癌发生之间的相关性.结果:polβ-SSCP结果显示癌旁组织标本中polβ无突变;26例鼻咽癌组织标本中存在polβ基因变异的有8例,突变率为30.8%.polβ基因变异的鼻咽癌标本测序结果显示:核苷酸序列有多种形式的改变.提取DNA用EBV引物进行扩增,26例鼻咽癌标本中出现阳性的有24例,阳性率为92.3%.结论:首次发现在鼻咽癌中存在多种形式的polβ突变,有polβ突变的病例均有EBV感染.

AIM： To study DNA polymerase β （polβ） gene mutation in human nasopharyngeal cancer and its relationship with EBV. METHODS： Twenty-six cases of nasopharyngeal carcinoma tissues and corresponding normal epithelial tissues were collected to study DNA polβ gene mutation with PT-PCR, PCR-SSCP and DNA sequence analyzing techniques. DNA was extracted and amplified with EBV primers and the results were observed and analyzed. RESULTS： polβ gene mutation was detected by SSCP in 8 of the 26 nasopharyngeal cancer tissues （with mutation rate 30.8%） and there was no mutation in juxtacancerous tissues. Many types of mutation were found by DNA sequencing. EBV-DNA positive rate was 92.3%. The results of experiment indicated that the cases of polβ-SSCP abnormal were accompanied with EBV infection. CONCLUSION： There is polβ alternation in human nasopharyngeal carcinoma, which leads to the sequence alternation in amino acid and spatial structure change. These mutations and EBV infection may play an important role in tumor genesis.