PML-RARα多肽诱导脐血TCR VβT细胞增殖研究

Clonal Expansion of TCR Vβ Subfamily T Cells Induced by PML-RARα Peptide

目的了解PMLRARα多肽诱导脐血T细胞的T细胞受体(TCR)Vβ谱系表达和克隆性情况。方法以不同浓度(16.7μg/mL,33.3μg/mL或50μg/mL)的PMLRARα多肽联合IL2、抗CD3单抗以及抗CD28单抗诱导脐血T细胞增殖,利用RTPCR基因扫描技术分析诱导后第3、6、9、12天后的脐血TCRVβ亚家族的利用和克隆性增殖的特点。结果限制性表达和克隆性增殖TCRVβ亚家族T细胞可见于PMLRARα多肽诱导后脐血T细胞。结论PMLRARα多肽可在体外诱导脐血T细胞呈克隆性增殖,此优势增殖的克隆性T细胞可能具有特异性细胞毒作用。

Objective To investigate the expression and clonality of T cell receptor （TCR） Vβ repertoire in cord blood T cells induced by PML-RARα peptide in vitro. Methods Cord blood mononuclear cells were amplified by IL-2, anti-CD3 and anti-CD28 antibody with different concentration （16. 7μg /mL, 33. 3μg/mL or 50μg/mL respectively） of synthetic PML-RARα peptide. The induced T cells were collected at different time points after culture （3, 6, 9 or 12 days）. The expression and clonality of TCR Vβ subfamilies within induced T cells were analyzed by using RT-PCR and genescan technique. Results Restricted expression and cloanl expansion of TCR Vβ subfamily cord blood T cells could be identified after induction by PML-RARα peptide. Conclusion The PML-RARα peptide could induce the clonal expansion T cells from cord blood in vitro, which may have specific cytotoxicity for PML-RARα ＋ cells.