E-cadherin、β-catenin、FAK在大肠癌的表达及其意义

Expression of E-cadherin,β-catenin and FAK in Colorectal Carcinoma and Their Signi-ficances

目的探讨β连环素(βcat)、上皮钙黏附素(Ecad)和黏着斑激酶(FAK)在大肠癌发生、发展中的作用。方法采用免疫组织化学方法检测了12例正常大肠黏膜,28例大肠腺瘤及49例大肠癌组织的βcat、Ecad和FAK表达情况。结果大肠癌βcat异位表达率61.2%,显著高于大肠腺瘤(35.7%P<0.05)。大肠癌βcat、Ecad膜表达缺失率分别为67.3%,57.1%,显著高于正常大肠黏膜和大肠腺瘤(P<0.01,P<0.01)。大肠癌FAK阳性率65.3%,高于大肠腺瘤和正常大肠黏膜(P<0.01)。βcat异位表达、βcat和Ecad膜表达缺失、FAK表达与大肠癌的浸润程度、淋巴结转移和Dukes分期等因素有关。大肠癌中,FAK表达与βcat、Ecad膜表达缺失及βcat异位表达呈正相关。结论βcat异位表达可能参与了大肠癌的发生、发展。βcat和Ecad膜表达缺失、βcat异位表达、FAK表达可能与大肠癌的侵袭和转移有关,此过程中,FAK可能抑制了癌细胞之间的黏附。

Objective To investing ate the role of β-catenin, Kcadherin and FAK in the carcinogenesis and progression of colorectal carcinoma. Methods Expression of β-catenin, E-cadherin and FAK was exam- ined immunohistochemically in 12 cases of normal colorectal mucosa,28 cases of colorectal adenoma,49 cases of colorectal carcinoma. Results The rate of cytoplasmic and/or nuclear expression of β-catenin was 61.2% in coloretal carcinoma,which was significantly higher than that in colorectal adenoma（35. 7%, P （0. 05）. The rate of the reduced membranous expression of β-catenin and E-cadherin was 67. 30/00, 57. 1% respectively in colorectal carcinoma, which was significantly higher than that in the colorectal adenoma and normal colorectal epithelium. The rate of FAK expression was 65. 3% in colorectal carcinoma, which was significantly higher than that in the colorectal adenoma and in the normal colorectal epithelium （P〈0. 01 ）. In addition, the last four kinds of expression was associated with degree of soakage, lymph node metastasis and Dukes stage. There was a positive correlation between FAK expression and the re duced membranous expression of β-catenin might play a role in the carcinogenesis and progression of colorectal carcinoma. Conclusion The cytoplasmic and/or nuclear expression of β-catenin, the reduced membranous expression of β-catenin and E-cadherin and FAK expression might be related to the invasion and metastasis of colorectal carcinoma, In which FAK might restrain the adhesion among carcinoma cells.