小鼠组织中Cxcl15 mRNA表达及其在肺组织中的的定位特征

Cxcl15 mRNA expression in mouse tissue and localization in mouse lung

目的观察Cxc l15(chemok ine,C-X-C motif,ligand 15)基因在小鼠体内的表达分布规律。方法采用North-ern,将小鼠Cxc l15基因与小鼠8种主要组织来源的Poly A+RNA印迹膜进行杂交,观察该基因在小鼠各种组织中的分布规律;采用原位杂交,观察该基因在小鼠肺组织中的分布规律。结果Cxc l15基因主要在小鼠肺组织支气管上皮细胞胞浆中表达,并可能存在不同亚型。结论Cxc l15基因的表达存在组织特异性。

Objective To observe distribution characteristic of Cxcl15 gene in mouse. Methods The Northern blotting was applied to analyze the distribution characteristic of Cxcl15 gene in mouse tissue and In situ hybridization for the distribution characteristic of Cxcl15 gene in the mouse lung. Results Cxcl15 gene mainly expressed in cytoplasma of bronchial epithelium in the mouse lung and maybe have two different isoforms. Conclusion The expression of Cxcl15 gene was of tissue specificity.