Serrate1基因转染对小鼠树突状细胞生物学特性的影响

Effect of Serrate1 gene transfection on biological characteristic of dendritic cells in mice

目的探讨Serrate1基因转染对小鼠树突状细胞生物学特性的影响。方法将Serrate1基因体外转染小鼠DCs,经G418筛选后,W estern b lot检测DCsSerrate1的表达,流式细胞仪检测转染后DCs表面共刺激分子的表达,混合淋巴细胞反应观察DCs对T淋巴细胞增殖的影响。结果Serrate1基因转染的树突状细胞Serrate1的表达较未转染组明显增高,转染Serrate1基因的DCs表面CD80、CD86等共刺激分子的表达与对照组比较无明显改变(P>0.05);在混合淋巴细胞反应中,转染DCs对T细胞的增殖有抑制作用,而空载体pcDNA 3.1(+)对照组与未转染组的树突状细胞具有强烈的激发T细胞增殖的能力。结论Serrate1基因转染能在不影响DCs表面共刺激分子表达的情况下显著抑制T细胞活化。

Objective To investigate the effects of Serrate l gene transfer on the biological characteristic of dendritic cells （DCs） in mice. Methods Mice DCs were transfected with pcDNA3.1 （ ＋ ） -Serratel plasmid through Lipofectmine 2000 and the transfected cells were selected by G418. The biological characteristic of transfected DCs were detected by Western blotting, the expression of the costimulatory molecules on the transfected DCs were detected by FACS, and the proliferation of T cells were tested by mixed lymphocyte reaction （MLR）. Results The expression of Serratel on the transfected DCs increased significantly than that of non-transfected DCs. There was no difference in the expression of costimulatory molecules between the transfected DCs and non-transfected DCs. In MLR, the proliferation of T lymphocyte cells was inhibited by transfected DCs, but the control DCs stimulated the proliferation strongly. Conclusion DCs overexpressing Serratel could inhibit the proliferation and activation of T cells, which was not related to the expression of costimulatory molecules on DCs but via the Notchl-Serratel signaling pathway.