能量可控陡脉冲对小鼠U_(14)宫颈癌杀伤作用的实验研究

Lethal effects of energy controllable steep pulses on U_(14) cervical cancer in mice

目的观察能量可控陡脉冲对小鼠U14宫颈癌的杀伤作用。方法取雌性BALB/C小鼠48只,建立U14宫颈癌皮下移植瘤模型,分别观察实验组和对照组各时相的组织形态学变化,并对抑瘤曲线进行分析。结果通过组织学和抑瘤曲线检测证实:陡脉冲电场能选择性杀死肿瘤细胞,抑制肿瘤细胞的增殖。组织形态学改变在治疗后即刻光、电镜观察可见肿瘤细胞变性,核固缩、核碎裂、核溶解坏死等征象,并随时间推移坏死更加明显。结论能量可控陡脉冲对肿瘤具有杀灭和抑制作用。

Objective To investigate the lethal effects of energy controllable steep pulses on U14 cervical cancer in mice. Methods A total of 48 female BALB/C mice were inoculated subcutaneously with the solution of U14 cervical cancer cells line at the right hind limb. The histomorphological changes were observed and the suppressive curve of the tumor was analyzed between experimental group （ n = 26 ） treated with steep pulse stimulation and control group （n = 22 ） without any treatment. Results Steep pulses electrical field could selectively injure tumor cells and inhibit cell proliferation. In the experimental group, the phenomena of pyknosis, karyorrhexis and karyolysis of tumor cells were observed by light and electron microscope and got worse as time prolonged. Conclusion Energy controllable steep pulses have a lethal or inhibitory effects on cervical cancer cells.