RDP1258免疫调节功能在大鼠体内实验研究

Study of the immunomodulatory effect of RDP1258 in vivo

目的观察一种新型HLA衍生肽(RDP1258)的体内免疫抑制功能并探讨其机制。方法人工固相合成HLA衍生肽(RDP1258),以3H-TdR法观察其在体内对大鼠脾细胞增殖反应的影响;与HO-1酶活性激动剂HEM IN及拮抗剂ZNPP相对比,采用酶化学法及免疫组化方法观察其对体内脾细胞血红素氧合酶-1(hem e oxygenase-1,HO-1)活性的影响。结果RDP1258体内应用可显著抑制淋巴细胞转化及MLR的增殖反应;该肽能够明显提高体内HO-1活性,并增强HO-1的表达。结论RDP1258体内应用能够显著抑制大鼠脾细胞由丝裂原或同种抗原引起的增殖反应,这种作用可能是通过影响HO-1活性而达到的。

Objective To observe the immunosuppressive function of a novel HLA-derived peptide,RDP1258, and to investigate the mechanisms in vivo. Methods A peptide derived from HLA, RDP1258, was chemically synthesized. The effects of the peptide on alloreactive cytotoxic activities of rat spleen cells in vivo were observed using ZH-TDR method. As compared with the HO-1 inducer hemin and the HO-1 inhibitor zinc protoporphyrin （ZnPP）, the HO enzyme activity was analyzed by enzyme chemistry method. Results The in vivo administration of the synthetic HLA-derived peptide can obviously inhibit the proliferation of rat spleen cells stimulated by isoantigen and mitogen, and resulted in upregulation of splenic heme oxygenase activity and rapid upregulation of HO-1 protein expression. Conclusion RDP1258 inhibits the proliferation of rat spleen cells induced by mitogen and isoantigen probably through enhancing the HO-1 activity.