六种中药复方乙醇提取物对酪氨酸酶激活作用及动物致色素作用的研究

The Studiy on the Effects of Ethanol Extracts from six Traditional Chinese Herb Prescriptions on the Activity of Tyrosinase and Melanogenesis in Vivo and in Vitro

目的探讨中医理法和实验药效的关系及其可能的作用机制,为临床治疗白癜风优化组方提供实验依据。方法离体实验为中药复方乙醇提取物对酪氨酸酶的影响;动物实验选用棕色豚鼠为模型,观察中药致色素作用。结果Ⅰ方、Ⅱ方、Ⅲ方、Ⅵ方对酪氨酸酶有激活作用(P<0.05);6种中药复方均使豚鼠表皮基底层中含黑素颗粒细胞增多(P<0.05);Ⅱ方、Ⅲ方、Ⅵ方中药使豚鼠表皮内Dopa阳性细胞增多(P<0.05)。结论对酪氨酸酶具有激活作用的中药组成复方并不一定对酪氨酸酶有激活作用;中药复方在离体和活体上的作用并不完全平行,提示中药对黑素生成的机理,不仅限于对酪氨酸酶的激活作用。

Objective To explore the relationship between traditional Chinese theory and the efficacy of experimental drugs, and their possible mechanism, which provide scientific basis for optimal prescriptions in the treatment of vitiligo. Methods The effects of the traditional Chinese herb prescriptions on tyrosinase activity were studied in vitro. Brownish guinea pig was used as a model to observe effect of the traditional Chinese herb prescriptions on melanogenesis in vivo. Results The extracts from prescription Ⅰ ,Ⅱ , Ⅲ, Ⅵ increased the activity of tyrosinase significantly （ P 〈 0.05 ）. Studies on melanogenesis showed that all of six prescriptions increased the number of melanin containing cells and melanin granules in basal layer （ P 〈 0.05 ） , and prescription Ⅱ , Ⅲ, Ⅵ increased the number of dopa-positive melanocytes （ P 〈 0.05 ）. Conclusion The prescriptions made of traditional Chinese herbs may not have the same effect on tyrosinase as single component does. The effect of the drugs tested on melanogenesis in vivo was not completely parallel to the activative effect on tyrosinase activity in vitro, suggesting that the mechanism that the traditional Chinese herbs affect melanogenesis might not only be through the activation of tyrosinase.