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角蛋白17的置换肽的克隆表达及纯化 被引量：2

Gene Cloning,Expression and Purification of APL of Keratin 17
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摘要 目的克隆角蛋白17的置换肽(APL),表达并纯化置换肽。方法将人工合成的置换肽DNA片段连接至表达载体pGEX4T1,转化大肠杆菌,IPTG诱导表达融合蛋白,分析鉴定表达蛋白,并纯化蛋白。结果①连接到载体的DNA序列,序列测定证实与已知序列一致;②成功构建了融合表达载体;③表达了pGEX4TS12E融合蛋白;④得到纯化的融合蛋白。结论人角蛋白17的置换肽的克隆成功及融合蛋白的表达与纯化,为进一步探索置换肽的功能打下了基础。 Objective To clone APL of keratin 17 ,express and purify APL. Methods The DNA fragments were inserted into vector pGEX-4T-1. APL was expressed in E coli as fusion protein induced by IPTG. The fusion protein was purified. Results ①The DNA sequence was confirmed by sequencing;②Fusion expression vector was successfully constructed;③The fusion protein pGEX-4T-S12E was correctly expressed;④Pufified fusion protein was obtained. Conclusion We successfully cloned APL of keratin 17 and constructed fusion expressing vector. The fusion protein was correctly expressed in E coll. We purified the fusion protein. What we have done will surely be useful to the study of APL.
作者 史晓蔚 沈柱 刘玉峰
机构地区 第四军医大学西京医院皮肤科
出处 《中国皮肤性病学杂志》 CAS 北大核心 2005年第10期595-597,共3页 The Chinese Journal of Dermatovenereology
关键词 角蛋白 置换肽 克隆 银屑病 Keratin APL Clone Psoriasis
分类号 R392.11 [医药卫生—免疫学]
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参考文献9

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共引文献18

同被引文献10

  • 1沈柱,刘玉峰,王刚.以T细胞介导为主的自身免疫性疾病的干预策略[J].细胞与分子免疫学杂志,2005,21(B03):71-73. 被引量：6
  • 2史晓蔚,沈柱,樊建勇,党育平,王刚,刘玉峰.置换肽对豚鼠银屑病样模型表皮增殖的治疗作用[J].中国皮肤性病学杂志,2006,20(9):523-525. 被引量：2
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中国皮肤性病学杂志
2005年 第10期

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