蕈样肉芽肿患者皮损中朗格汉斯细胞的迁移研究

Study of the migration of dendritic cells in the skin infiltrates of MF patients

目的:探讨蕈样肉芽肿(MF)患者皮损中皮肤淋巴细胞相关抗原(CLA)、CD62L、共刺激分子(CD40、CD80、CD86)分布及其在树突状细胞(DC)上的表达。方法:将MF皮损作冰冻切片,行单克隆抗体免疫组化染色。结果:肿瘤组织真皮及表皮内可见大量CLA、CD62L、主要组织相容性复合体(MHC)Ⅱ、共刺激分子表达,CLA的表达细胞明显多于CD62L,由斑块期到肿瘤期,表皮内CLA表达数目明显减少,真皮中CLA、CD62L表达增强。CD83+DC能表达强的共刺激分子,在基底层、真皮和血管内可见CD83+DC表达CD62L,但未成熟DC常表达CLA。结论:MF皮损中朗格汉斯细胞发生迁移,参与了抗肿瘤免疫反应。

Objective： To identify the expressive situation of cutaneous lymphocyte-associated antigen（CLA）, CD62L,MHC II and co-stimulate molecule in the skin infiltrates of MF patients and dyeing expression of those molecules on the surface of dendritic cells（DCs）. Methods： Mab and immunohistochemistry were used to detect the expression of special antigen on the surface of DC in situ. Results： The equal numbers of CLA, CD62L, MHC II and co-stimulate molecules were expressed in the skin infiltrate of MF. The numbers of CLA~ cells were more than CD62L^＋ cells, but from plaque stage to tumor stage, the numbers of CLA^＋ cells in the epidermis decreased and the expression of CLA and CD62L were enhanced in the dermis. CD83^＋ DCs could strongly expressed co-stimulate molecules. The CD83^＋ DCs expressing CD62L were easily seen in the basal layer, in the dermis and in the vascular, but immature DCs expressed often CLA molecules. Conclusion： Langerhans cells in the skin lesions could migrate and induce an anti-tumor immune response in MF.