脑源性神经营养因子在鸡形觉剥夺性近视恢复期中的作用

Action of brain-derived neurotrophic factor in the convalescent of form deprivation myopia in chicken

目的观察形觉剥夺性近视(FDM)恢复期中视网膜脑源性神经营养因子(BDNF)水平的变化,探讨BDNF在FDM中的作用。方法鸡雏单眼遮盖建立FDM模型,剥夺眼去遮盖恢复2周,应用免疫组织化学技术检测剥夺眼视网膜神经节细胞层BDNF的表达。结果去遮盖2周后,剥夺眼BDNF阳性细胞数增加。结论形觉剥夺眼去剥夺后,随着近视程度的减轻,视网膜BDNF的表达水平升高。

Objective To observe the expression of brain-derived neurotrophic factor （BDNF） on the form deprived chicken retina in myopia convalescent. Methods Model of form deprivation myopia was established in chicken by monocular occlusion. The goggle was removed and chicken continued to be raised for two weeks. Expression of BDNF on the model chicken retina was measured using immuonhistochemieal method. Results Model of myopia was created following the 2-week ocular occlusion with the diopt（ - 18. 427 5 ± 1. 437 7） ,and the diopt was reduced after 2-week de-occlusion（ - 8. 468 8 ± 1. 270 8）. Immunohistochemical staining showed that the numbers of positive cells in retina from the de-occluded eyes were （24. 600 0 ± 0. 320 7 ） and those of the control eyes were（ 26. 475 0 ± 0. 861 5 ） and（ 13. 975 0 ± 0. 712 6 ） in 2-week occlusion eyes, suggesting a significant decrease in the number of positive cells in de-occlusion eyes compared to control eyes （ P 〈 0.01 ） and a lowest staining number in the retina of deprived eyes（ P 〈 0.01 ）. The expression of BDNF in the ganglion cell layer was increased after goggle removed,but its expression level in the occluded eye was still lower than that of the control one with a significant difference between them（P 〈0. 001 ）. Conclusion Form deprivation reduces expression of BDFN in retina, and this status is improved with the remove of deprivation.