玻璃化冷冻人早期胚胎的效果观察

Vitrification of human cleaved embryos in vitro fertilization-embryo transfer

目的探讨玻璃化冷冻人早期胚胎的临床应用价值。方法采用由5·5mol/L乙二醇及1·0mol/L蔗糖制成的冷冻保护液,于0·25ml冷冻麦管中,冷冻经体外受精-胚胎移植第2天或第3天后剩余的胚胎;对新鲜周期胚胎移植后未妊娠的胚胎,至少3个月或3个月以上施行胚胎复苏,观察胚胎复苏后的临床妊娠效果。结果共行胚胎复苏219例,957个胚胎,胚胎复苏后存活691个,胚胎存活率为72·2%(691/957);移植胚胎178例,临床妊娠35例,临床妊娠率为19·7%(35/178)。35例中,分娩16例,流产6例,继续妊娠13例。结论玻璃化冷冻法简便,胚胎存活率高,是一种可用于临床的人早期胚胎冷冻的方法。

Objective To assess the outcome of thawing human cleaved embryos from cryopreservation by vitrification. Methods A total of 957 day 2 or day 3 embryos from 219 patients were thawed after vitrification with ethylene glycol 5.5 solution and 0. 25 ml straw between Jan 2003 and Jun 2005,514 embryos were recovered and transferred in 178 patients. Results The survival rate of thawing embryos and the clinical pregnancy rate after transfer was 72. 2% and 19. 7% respectively. Twenty-two healthy babies were born from 16 deliveries, including 12 girls and 10 boys, 6 pregnancies ended in miscarriage and another 13 are ongoing. Conclusion Vitrification method is an alternative for cryopreservation of human cleaved embryos because of high effectiveness, convenience and good cost efficiency.