罗哌卡因对映异构体的三甲基-β-环糊精HPCE手性拆分研究

Chiral Separation of Ropivacaine by Capillary Electrophoresis with Heptakis (2,3,6-tri-O-methyl)-β-cyclodextrin

目的:采用毛细管电泳法对罗哌卡因进行手性分析比较。方法:以30 mmol·L^(-1)三甲基-β-环糊精(TM-β-CD)为手性选择剂,0.2 mol·L^(-1)磷酸-三乙醇胺(pH 3.5)缓冲溶液为背景电解质,+20 kV,20℃,206 nm 波长检测,对罗哌卡因进行毛细管电泳法手性分离分析,探讨了多种因素对分离的影响。结果:在本文确定的对映体纯度试验条件下,对映体可基线分离。结论:以三甲基-β-环糊精为手性选择剂的毛细管电泳法方便易行,可用于罗哌卡因对映体纯度检查。

Objective：Chiral separation of ropivacaine enantiomers by capillary electrophoresis using heptakis （2, 3,6 -tri- O- methyl） -β -cyclodextrin as chiral resolving agent were studied. Methods： The enantiomers were separated by HPCE with 30 mmol · L^-1 heptakis（2,3,6 -tri -O- methyl） -β -CD as the chiral resolving additive in O. 2 mol · L^-1 phosphric acid - triethanolamine background electrolyte at pH 3. 5 on a capillary of 42.0/50. 5 （I/L）cm × 50 μm ,and positive voltage of 20 kV of electric force was used with the cassette temperature set at 20 ℃ and UV detection at 206 nm. Results：The enantiomers were baseline separated at the established conditions for the enantiomeric purity test of S -ropivacaine. Conclusions ：The established CE method is of high performance, ruggedness, and economv for the chiral senaration and routine nuritv test of ronivacaine.