中药槐花饮片特征性提取物B的指纹图谱研究

Studies on Fingerprints of Characteristic Extracts B of Processed Pieces of Flos Sophorae

目的：采用^1HNMR法和HPLC法研究中药槐花饮片特征性提取物B的指纹图谱，探讨炮制对槐花中特征性成分的影响。方法：按照规范工艺制备槐花、炒槐花和槐花炭的特征性提取物B（SCE B），分别测定各饮片的^1HNMR和HPLC指纹图谱。参照从槐花炭中分离得到的单体化合物（compound 1～5）的^1HNMR谱，分析3种饮片SCEB的^1HNMR谱，得到特征性成分的整体信息。以各单体成分为对照品分析3种饮片SCEB的HPLC指纹图谱，得到芳香类成分的信息。结果：3种饮片SCEB的^1HNMR谱基本一致，均显示主要为皂苷类成分的信息，还有少量芳香类成分的信息，表明炮制后其特征性成分未发生显著性变化。3种饮片的HPLC指纹图谱显示炒槐花与槐花的共有峰数目及各峰保留时间一致，而槐花炭中出现1个新的色谱峰，经对照品对照为化合物3，表明槐花制炭后有微量新成分产生。结论：中药槐花饮片的^1HNMR和HPLC指纹图谱分别反映了槐花炮制前后特征性成分的不同结构和整体组成，可作为其基源鉴定及炮制品鉴别的参考。

Objective：To study the fingerprints of the processed pieces of Flos Sophorae by ^1H NMR and HPLC;to explore the effect of processing on Flos Sophorae. Methods： The standard characteristic extracts B （ SCE B） of Flos Sophorae, Flos Sophorae Tostus and Flos Sophorae Carbonisatum were obtained according to a standardized procedure. Their ^1H NMR and HPLC fingerprints were determined,separately. Comparing with the ^1H NMR spectra of pure compounds 1 -5 from Flos Sophorae Carbonisatum, the main characteristic signals in the ^1H NMR fingerprints of SCE B of the processed pieces of Flos Sophorae were assigned. And comparing with those compounds, the benzene circles signals in HPLC fingerprints of SCE B of the three processed pieces were obtained. Results：The ^1H NMR fingerprints of the SCE B of the three processed pieces showed identical, which were main saponins signals and trifle benzene circles signals. It revealed the main characteristic compounds hadn＇t changed significantly after processing. It showed the number and retentive time of the common peaks of Flos Sophorae Tostus were as same as Flos Sophorae. Flos Sophorae Carbonisatum appeared a new peak, and it was compound 3. This revealed new trifle compound produced after carbonized. Conclusion：It showed the structure and holistic constitute of characteristic constituents of Flos Sophorae afte.r processing according to the fingerprints of SCE B of those processed pieces by ^1H NMR and HPLC, separately. They can be utilized for the original authentication of this plant species and the identification of processed varieties.