大剂量黄芪对急性白血病患儿外周血树突状细胞的诱导和抗原呈递功能的影响

Effects of Large Dose of Astragalus membranaceus on the Dendritic Cell Induction of Peripheral Mononuclear Cell and Antigen Presenting Ability of Dendritic Cells in Children with Acute Leukemia

目的观察大剂量黄芪对急性白血病患儿外周血单核细胞体外诱导扩增为树突状细胞(DC)及对DC抗原呈递功能的影响.方法44例急性白血病完全缓解期患儿分为黄芪组与对照组,黄芪组20例,对照组24例.黄芪组于常规化疗同时应用大剂量黄芪(90g/d)1个月,对照组仅常规化疗.提取两组外周血单个核细胞(MNC),贴壁获得单核细胞,加入白细胞介素-4(IL-4)、粒细胞巨噬细胞集落刺激因子(GM-CSF)、肿瘤坏死因子(TNF-α)等细胞因子培养7～8天,利用流式细胞仪进行DC的表型鉴定,用混合淋巴细胞反应鉴定DC的抗原呈递能力.结果黄芪组与对照组从外周血单核细胞诱导生成的DC形态学差异无显著性;黄芪组DC的细胞数量平均为4.4×106/2.5×106MNC,对照组DC的数量平均为2.6×106/2.5×106MNC,两组比较差异有显著性(P＜0.001);黄芪组DC可较强地刺激同种异体淋巴细胞增殖,与对照组比较差异有显著性(P＜0.001).结论大剂量黄芪可提高急性白血病患者外周血单核细胞诱导生成DC的数量并增强DC的抗原呈递功能.

Objective To explore the effects of large dose of Astragalus membranaceus （Astragalus） on the dentritic cell （DC） induction in vitro and augumentation by peripheral mononuclear cell （MNC） and on antigen presenting ability of DC in children with acute leukemia. Methods Forty-four children with acute leukemia in complete remission stage were divided into two groups. Twenty patients in the Astragalus（90g daily） group were treated with large dose of Astragalus（90g daily） based on conventional chemotherapy for one month, while 24 patients in the control group received chemotherapy alone. MNC were extracted from peripheral blood by wall-sticking method and cultured with such cell factors as interleukin-4,gramulocyte macrophage colony stimu- lating factor, tumor necrosis factor-α for 7 - 8 days. Phenotype of DC was assayed by flow cytometry and antigen presenting ability of them was assayed by mixed lymphocyte reaction. Results There was no morphological difference in MNC induced DC between the two groups. The trol group was 4.4× 10^6/2.5 × 10^6 MNC and 2.6 × 10^6/2. 5×10^6MNC, respectively,showing significant difference（P 〈 0.001 ）. DC in Astragalus group could stimulate the proliferation of allogeneic lymphocytes strong- ly, showing significant difference when compared with that in the control group （P 〈 0.001 ）. Conclusion Large dose of Astragalus could increase the I）C induction of MNC and enhance the antigen presenting ability of DC in acute leukemia patients.