IGF-I、TGF-α、bFGF对猪卵母细胞体外成熟和卵裂的影响

Effects of IGF-I,TGF-a,bFGF on Porcine Oocytes Maturation in vitro and Oocytes Cleavage

研究了IGF-I、bFGF、TGF-α3种细胞因子单独或者联合作用对猪卵母细胞体外成熟和孤雌激活后卵裂的影响,以确立猪卵母细胞体外成熟的最佳条件。结果表明,3种细胞因子对卵母细胞的成熟和激活后卵裂呈现双重效应(1)20ng·ml-1的IGF-I的成熟率和卵裂率显著的高于对照组和10、30、40、50、100ng·ml-1组[(85.3±2.11)%,(85.4±2.81)%;(71.1±1.91)%,(62.5±0.98)%;(77.5±2.5)%,(59.1±3.93)%;(61.9±1.72)%,(54.3±3.48)%;(58.6±4.26)%,(53.1±1.23)%;(44.4±5.10)%,(49.8±3.55)%;(33.9±3.48)%,(46.1±3.59)%,P<0.05),当IGF-I的浓度达到100ng·ml-1时,成熟率(33.9±3.48)%和卵裂率(46.1±3.59)%降低,与其它各组相比差异显著(P<0.05)。(2)20ng·ml-1的bFGF的成熟率(85.0±1.70)%和卵裂率(85.0±2.82)%最高,和其它各组相比,差异显著(P<0.05)。(3)15ng·ml-1的TGF-α的成熟率(86.9±0.46)%和卵裂率(86.3±2.01)%最高,和其它各组相比,差异显著(P<0.05)。(4)15ng·ml-1TGF-α和20ng·ml-1bFGF联用组,卵母细胞成熟率和分裂率显著高于20ng·ml-1bFGF和20ng·ml-1IGF-I联合组;15ng·ml-1TGF-α和20ng·ml-1IGF-I联合组;与20ng·ml-1bFGF、20ng·ml-1IGF-I和15ng·ml-1TGF-α三者联用组。[(89.2±1.44)%和(88.8±0.17)%.(75.6±0.98)%和(78.3±1.65)%;(77.2±2.54)%和(80.2±2.26)%;(76.4±1.28)%和(77.4±3.73)%,P<0.05]。

Effects of IGF-I, bFGF, TGF-α separation or combination on porcine oocytes maturation in vitro and cleavage after parthenogenetic activation were studied. The results showed that ： （1）The maturation rate and the cleavage rate derived from oocytes matured in medium supplemented with 20 ng·ml^-1 IGF-I is significantly higher compared with control and 10, 30, 40, 50, 100 ng·ml^-1 groups[（85.3±2.11）%, （85.4±2.81）%; （71.1±1.91）%, （62.5 ±0.98）%; （77.5±2.5）%, （59.1±3.93）%; （61.9±1.72）%, （54.3±3.48）%; （58.6±4.26）%, （53.1±1.23）%; （44.4±5.10）%, （49.8±3.55）%; （33.9±3.48）%, （46.1±3.59）%, P〈0.05], however when IGF-I solution arrive to 100 ng·ml^-1, the maturation rate and the cleavage rate is greatly significant lower than other group （P〈0.05）. （2）The maturation rate （85.0±1.70）% and the cleavage rate （85.0±2.82）% derived from oocytes matured in medium supplemented with 20 ng.mr1 bFGF is greatly significant higher than other group （P〈0.05）; （3） The maturation rate （86.9±0.46）% and the cleavage rate （86.3±2.01）% derived from oocytes matured in medium supplemented with 15 ng·ml^-1 TGF-α is greatly significant higher than other group （P〈0,05）; （4） The maturation rate and the cleavage rate derived from oocytes matured in medium supplemented with 15 ng·ml^-1 TGF-α and 20 ng·ml^-1 bFGF is significantly higher compared with 20 ng·ml^-1 bFGF and 20 ng·ml^-1 IGF-I group; 15 ng·ml^-1 TGF-ct and 20 ng·ml^-1 IGF-I group, 20 ng·ml^-1 bFGE 20 ng·ml^-1 IGF-I and 15 ng·ml^-1 TGF-α combatlon group.[（89.2±1.44）% and （88.8±0.17）%. （75.6±0.98）% and （78.3±1.65）% ; （77.2±2.54）% and （80.2±2.26）%; （76.4±1.28）% and （77.4±3.73）%, P〈0.05].