265例HIV-1抗体阳性者抗体蛋白印迹带型分析

Assay of western blot bands pattern of antibody of 265 samples HIV-1 antibody positive

目的通过对本实验室以蛋白印迹(WB)法确认的HIV-1抗体阳性者抗体WB条带的分析,了解不同人群抗体WB带型分布的差异。方法WB法确认HIV-1抗体阳性。结果抗pol基因编码蛋白的抗体WB带型分布以及P31抗体的阳性率在不同传播途径的HIV-1抗体阳性者间差异有统计学意义(P<0.01);年龄(age,A)≤40岁的HIV-1抗体阳性者P66和P24抗体阳性率分别明显高于相应A>40岁以上者,(P<0.05);抗pol基因编码蛋白的抗体WB带型分布以及P66抗体阳性率在不同地域的HIV-1抗体阳性者间差异有统计学意义(P<0.01)。结论HIV-1抗体WB带型分布在不同性别、不同年龄人群间差异无统计学意义,但在不同传播方式、不同地域的人群间差异有统计学意义;HIV-1 pol、gag基因编码的某些蛋白的抗体产生在不同传播方式、不同年龄及不同地域的人群间差异有统计学意义。

Objective To explore the difference of western blot （WB） bands pattern in various HIV-1 polsitive people by investigate the WB bands of HIV-1 antibody. Methods People diagnosed as HIV-1 posotive person by WB. Results The WB bands pattern of antibody resist antigen coded by pol gene and the positive rate of P31 antibody has significant difference between the people infected by different transmission mechanism, P 〈 0.01 ; The positive rate of P66 and P24 antibody of people less than or equal to 40 years was significant greater than that of people older than 40 years, P 〈 0.05; The WB bands pattern of antibody resist antigen coded by pol gene and the positive rate of P66 antibody has significant differnce among people from various regions. Conclusion HIV - 1 antibody WB bands pattern is not significant diffferent among people who are different sex or age, but it is different among people who are infected through different transmission mechanism or from various region; the production of some antibody resist antigen coded by HIV-1 pol or gag gene is different among peo- ple who are infected through different transmission mechanism or from various region or different age.