高剂量碘对小鼠T淋巴细胞免疫功能的影响

Effect of excess dose potassium iodate on immune function of T lymphocyte in mice

目的通过观察不同剂量碘酸钾作用下小鼠T淋巴细胞数量及亚群比值的变化探讨碘酸钾对淋巴细胞免疫功能的影响。方法采用80只BaLB/c雌性小鼠,按体重随机分4组,每组20只,每组分2批,10只为一批,分2批处理。不同剂量碘酸钾加入自来水中使其分别含碘1 500,3 000,6 000μg/L,自来水作为对照,将它们以自由饮水的方式分别喂饲4组小鼠,30 d后测定小鼠T淋巴细胞增殖反应能力,T细胞表面主要辅助分子CD4+、CD8+比值的变化,CD4+T细胞Th1,Th2比值的变化,杀伤细胞(NK)活性。结果含碘6 000μg/L组的CD4+/CD8+与正常对照组相比降低,淋巴细胞增殖反应能力、NK细胞活性与正常对照组相比升高,差异有统计学意义。结论小鼠饮水中含碘6 000μg/L时可造成淋巴细胞免疫功能紊乱。机体存在自体免疫性疾病发生的危险。

Objective Through investigating the variations of lymphproliferation response and ratios of subgroup, to approach the effect of excess dosage potassium iodate on immune function of T lymphocyte. Methods 80 female BaLB/c were divided into 4 groups randomly according to their body weight, 20 for each group, 10 of each group was considered as one batchfor test. mice in the 4groups were orally administrated including iodine 0, 1500, 3000, 6000μg/L, respectively for 30 days. they were used to observe lymphproliferation response, CD4^＋/CD8^＋, Th1/Th2, the activity of natural-killer cells （NK）.Resuits On the 6000μg/L, CD4^＋/CD8^＋ was significant lower than control＇s. lymphproliferation response, Th1/ Th2, the activity of natural-killer cells（NK） were significant higher than that of control. Conclusion Potassium iodate is exogenous chemical materials related to immune reaction. The dosage 6 000μg/L can induce disorders of immune function of T lymphocyte of mice. It may have a central role in the development of autoimmune disease.