休克大鼠血管外膜阳离子氨基酸运体表达

Expression of cationic amino acid transporter in adventitia of rats with sepsis

目的观察败血症休克大鼠主动脉外膜阳离子氨基酸转运体(CAT-1、CAT-2B)和诱导型一氧化氮合酶(iNOS)基因表达。方法雄性Wistar大鼠盲肠结扎并穿孔复制败血症休克模型。逆转录-聚合酶链(RT-PCR)方法测定诱导型一氧化氮合酶(iNOS)、CAT-1、CAT-2B mRNA表达水平。结果败血症休克大鼠表现为严重的心功能降低、低血糖和高乳酸血症。血管外膜CAT-1、CAT2B、和iNOS的mRNA水平均比假手术组明显上调(均P<0.01)。结论败血症休克时血管外膜阳离子氨基酸转运体和诱导型一氧化氮合酶基因表达上调。

Objective To assess the gene expression of cationic amino acid transporter-1（ CAT-1 ）, CAT-2B and inducible nitric oxide synthase（iNOS）in the adventitia of rats with sepsis. Methods Sepsis was induced by cecal ligation and puncture （CLP）as described with minor modification, And to observe iNOS gene expresion and activity and NO generation, cationic amino acid transporter（CAT-1, CAT-2B）gene expression to explore the mechanism of activation of L-Arg/NOS/NO pathway. Results Cecal ligation and puncture induced severe sepsis with severe low glucose and high lacticemia, The CAT-1 and CAT-2B mRNA level in the sepsis group was increased by 86% and 62% （both P〈0.01）, respectively. The iNOS mRNA level was elevated（P 〈 0.01 ）, The NO level in plasma and incubation media（ incubation for 40 min）in the sepsis group was increased by 144 % and 273 % （both P 〈 0.01 ）, respectively, Conclusion The up-expression of high - affinity CAT-1, CAT-2B and iNOS in vascular adventitia of rats with sepsis shock. Transcription of CAT-1 and CAT-2B correlates well with aortic NO biosynthesis.