血清HCVRNA荧光定量与丙型肝炎诊断意义

Significance of fluorescent quantitative serum HCVRNA and diagnoses in hepatitis C

目的探讨血清HCVRNA荧光定量在丙型肝炎诊断中的意义。方法采用ELISA法和荧光定量PCR法检测292例慢性丙肝患者抗-HCV和HCVRNA。结果抗-HCV及HCVRNA检出率分别为71.9%(210/292)和51.3%(150/292);抗HCV(+)组和抗-HCV(-)组HCVRNA的检出率分别为75.9%(82/108)和10.5%(4/83)。结论抗HCV(+)组HCVRNA检出率高于抗-HCV(-)组;血清HCVRNA荧光定量是临床诊断丙型肝炎的直接证据;抗-HCV诊断丙肝感染有漏诊现象。

Objective To explore the significance of serum levels of HCVRNA in hepatitis C diagnoses. Methods The anti-HCV was monitored by indirect enzyme-linked immunosorbent assay（ELISA）and serum levels of HCVRNA quantities were tested by fluorescent quantitative PCR（FQ-PCR）in 292 cases of chronic hepatitis C patients. Results The positive rates of anti-HCV and HCVRNA were 71.9 % （210/292）and 51.3 % （150/292）, respectively. The rates of HCVRNA in positive anti-HCV group and negative group were 75.9% （82/108）and 10.5% （4/38）, respectively. Conclusion The monitored rate of HCVRNA in positive anti-HCV group patients is higher than that of negative group. Serum levels of HCVRNA by FQ-PCR quantities are one of the best direct marks in diagnoses for gepatitis C that could make up for the missed diagnoses with the mark of anti-HCV.