创伤性脓毒症过程中外周血PMN及组织中ICE mRNA表达的变化

The changes of ICE mRNA expression in systemic blood PMNs and tissues during tramatic sepsis

目的:探讨盲肠结扎穿孔术(CLP)后大鼠外周血多形核白细胞(polymorphonuc lear leukocyte,PMN)及组织中白细胞介素1β转换酶(interleuk in-1βconverting enzym e,ICE,现称caspase 1)mRNA表达的变化规律。方法:采用逆转录多聚酶链式反应(reverse transcription-polym erase chain reaction,RT-PCR)检测大鼠外周血PMN及组织中ICE mRNA的表达。结果:CLP术后循环血中多形核白细胞(PMN)中ICE mRNA表达逐渐增高,达峰值后开始降低,但仍明显高于正常。ICE mRNA表达的增高首先出现于血PMN中,迟后转移至肺、小肠和肝组织中。结论:ICE mRNA表达的增高在脓毒症的发病机制中起着重要的作用。

Objective：To evaluate the changes of ICE mRNA expression in systemic blood PMNs and tissues after CLP in rats. Methods：To adopt RT-PCR to detect ICE mRNA expression in systemic blood PMNs and tissues. Results：The ICE mRNA expression in systemic blood PMNs rised gradually after CLP, it began to decrease after reaching peak, but higher than normal. It arised in systemic blood PMNs and transfered to lungs, small intestines and livers later. Conclusions ：The rise of ICE mRNA expression acts as an important role in sepsis pathogenesis.