灯盏花素对肝缺血再灌注损伤生化指标的影响

Effect of breviscapine on biochemistry in hepatic ischemia-reperfusion injury

目的:研究灯盏花素对肝缺血再灌注损伤生化指标的影响。方法:健康家兔32只,复制肝缺血再灌注损伤模型。随机分为假手术组,模型组,灯盏花素(B re)小剂量用药组和灯盏花素大剂量用药组,每组8只。观察缺血前,缺血45m in、再灌注30和60m in时血清超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-PX)、谷丙转氨酶(ALT)活性和丙二醛(MDA)含量及灯盏花素对不同时限上述指标的影响。结果:灯盏花用药组的SOD、MDA、GSH-PX和ALT在再灌注的各时限与对照组比较均有显著或非常显著差异(P<0.05或P<0.01)。结论:灯盏花素能通过抑制氧自由基的生成,增强氧自由基的清除,对肝缺血再灌注损伤起着良好的抗脂质过氧化作用。

Objective：To explor the role of breviscapine （Bre） on dynamic changes of lipid peroxidation in hepatic ischemiareperfusion injury （HIRI）. Methods：The HIRI model was used. Thirty-two rabbits were randomly divided into control group （n =8）, model group（n =8）, Bre （5mg） group （n =8） and Bre （7mg） group（n =8）. The superoxide dismutase （SOD） ac- tivity, malondialdehyde （MDA） content, glutathione peroxidase （GSH-PX） and alanine aminotransferase （ALT） activity in ser- um were observed before ischemia and at ischemia 45 min, reperfusion 30min, reperfusion 60 min. Results：The SOD activity, GSH-PX activity MDA, content and ALT activity of Bre group, as compared with model group, showed significant differences （P 〈0.05 or P 〈0.01 ） at total time points of reperfusion. Conclusion：Bre has notable anti-lipid peroxidation effect on HIRI, which is due to its inhibiting the generation of oxygen free radicals and its strengthening scavenging of oxygen free radicals.